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| Zakhour, Maha; Maalouf, Haifa; Di Bartolo, Ilaria; Haugarreau, Larissa; Le Guyader, Francoise; Ruvoen-clouet, Nathalie; Le Saux, Jean-claude; Ruggeri, Franco Maria; Pommepuy, Monique; Le Pendu, Jacques. |
| Noroviruses (NoV) are major agents of acute gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Previous studies showed that some human strains bind to oyster tissues through carbohydrate ligands that are similar to their human receptors. Thus, based on presentation of shared norovirus carbohydrate ligands, oysters could selectively concentrate animal strains with increased ability to overcome species barriers. In comparison with human GI and GII strains, bovine GIII NoV strains, although frequently detected in bovine feces and waters of two estuaries of Brittany, were seldom detected in oysters grown in these estuaries. Characterization of the carbohydrate ligand from a new GIII strain indicated recognition of the... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12534/9527.pdf |
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| Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. |
| The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... |
| Tipo: Text |
Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. |
| Ano: 2000 |
URL: https://archimer.ifremer.fr/doc/00507/61879/65953.pdf |
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| Dubois, E; Le Guyader, Soizick; Haugarreau, Larissa; Kopecka, H; Cormier, M; Pommepuy, Monique. |
| Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples, Amplified viral sequences were analyzed bg restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples, The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were... |
| Tipo: Text |
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| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00336/44720/44851.pdf |
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| Dupraz, Valentin; Coquille, Nathalie; Menard, Dominique; Sussarellu, Rossana; Haugarreau, Larissa; Stachowski-haberkorn, Sabine. |
| A wild strain of Chaetoceros calcitrans and wild and diuron-resistant strains of Tetraselmis suecica, were exposed to the PSII inhibitor herbicides diuron and irgarol, individually and in mixtures. The effects of three concentrations of diuron and irgarol and four binary mixtures were evaluated on doubling time, relative reactive oxygen species and lipid content by flow cytometry, and on photosynthetic efficiency by pulse amplitude modulated fluorescence. In both wild strains, significant effects were observed for each molecule at the highest concentration tested: at irgarol 0.5 μg L−1, C. calcitrans was shown to be more sensitive than T. suecica (+52% and +19% in doubling time, respectively), whereas at diuron 5 μg L−1, T. suecica was more affected... |
| Tipo: Text |
Palavras-chave: Microalgae; Herbicides; Antifouling; Irgarol; Diuron; Diuron resistance. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00319/43047/42658.pdf |
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| Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. |
| The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... |
| Tipo: Text |
Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-1363.pdf |
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| Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique. |
| Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between... |
| Tipo: Text |
Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf |
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| Miossec, Laurence; Besseau, Laetitia; Haugarreau, Larissa; Le Guyader, Francoise; Menard, Dominique; Pommepuy, Monique; Abadie, Eric; Comps, Marie-annick; Guillou, Jean-louis; Le Bec, Claude; Sauvagnargues, Jean-claude; Bon, Fabienne; Jamot, B.; Kohli E, Evelyne; Potier, P.; Daurat, G.; Guyonnet, J.p.. |
| Les microorganismes, bactéries et virus d'origine entérique, sont abondants dans les rejets urbains. Si la charge bactériologique contenue dans les eaux usées est notablement réduite lors des traitements appliqués dans les stations d'épurations, de nombreux microorganismes persistent et sont rejetés avec les eaux épurées dans le milieu récepteur, en rivière ou directement en mer. Le développement de méthodes analytiques basées sur la biologie moléculaire a permis récemment la détection de virus entériques non cultivables dans les échantillons de l'environnement, tels que les entérovirus, le virus de l'hépatite A, les calicivirus, les astrovirus et les rotavirus. En France, les données publiées sur la présence de ces virus entériques, détectés par RTPCR,... |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00044/15552/12941.pdf |
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