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| He,L; Xu,H; Ye,F; Yu,H; Lu,Y; Yin,H; Zhao,X; Zhu,Q; Wang,Y. |
| ABSTRACT Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. HSPGs interact with growth factors and receptors through heparan sulfate (HS) chains. The sulfation pattern of heparan sulfate chains influences signaling events mediated by heparan sulfate proteoglycans located on the cell surface. SULF1 and SULF2 are two endo-sulfatases that can cleave specific 6-O-sulfate groups within the heparan chains. To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. The SULF1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; SULF1; SULF2; Satellite cell; Immunocytochemistry. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300307 |
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| Cui,C; Ye,F; Li,Y; Yin,H; Ye,M; He,L; Zhao,X; Xu,H; Li,D; Qiu,M; Zhu,Q; Wang,Y. |
| ABSTRACT BMP6, a member of the subfamilies of the morphogenetic proteins (BMPs), plays a crucial role in osteogenic and chondrocyte differentiation in vitro and stimulates chondrogenesis, making chondrocytes differen-tiate on their terminal stage. The objective of this study is to explore the relationship between polymorphism of BMP6 gene and slaughter traits in chicken respectively. We screened the exonic and intronic regions of BMP6 gene by DNA pool construction and amplified DNA fragment by PCR, and finally, we got nine SNPs. Association analysis revealed that BMP6 had no significant association among all slaughter traits in Yellow bantam chicken. However, BMP6 had a significant difference with femur weight, tibia weight, femur length (p<0.05), and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BMP6 gene; Bone traits; Carcass traits; Chicken; Polymorphisms. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400673 |
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| Yang,F; He,L; Luo,B; Ye,F; Cui,C; Yu,X; Xu,H; Zhao,X; Yin,H; Li,D; Zhu,Q; Wang,Y. |
| ABSTRACT There is increasing evidence that bone morphogenetic protein 6 (BMP6) plays critical roles in regulating various stages of ovarian follicle development in mammals. However, the mechanisms of regulation of BMP6 in the chicken ovary remain unclear. In this study, mRNA and protein expression level of BMP6 in chicken ovarian follicles at different development stages were determined by qRT-PCR and western blot separately. Different concentrations of BMP6 protein and FSH were added to the culture medium, and the effects to proliferation of granulose cells were detected, further effect on expression pattern of progesterone synthesis associated genes were also analyzed by qRT-PCR and Western blotting and the secretion of progesterone was detected by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; BMP6; Granulosa cells; Progesterone; FSH. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200310 |
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