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| Chen,Xiwen; Chen,Wenchuan; He,Yun; Zhang,Yixin; Chen,Chenfeng; Zhu,Zhimin; Wang,Hang. |
| ABSTRACT We previously revealed the involvement of extracellular regulated protein kinases 1/2 (ERK1/2) in interleukin-6 (IL-6) secretion induced by cyclic compressive force (CCF) in MLO-Y4 cells. In this study, we investigated the contributions of the p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways to IL-6 secretion by stimulating MLO-Y4 cells with CCF. At 80% confluence, different magnitudes (1000μstrain, 2000 μstrain and 4000 μstrain), frequencies (0.5 Hz, 1.0 Hz and 2.0 Hz) and durations (10 min, 30 min, 1 h, 3 h and 6 h) of CCF were loaded onto cells using a four-point bending system. Flow Cytometry (FCM) analysis was used to analyze cell mortality rates after CCF loading. p38 and p65 phosphorylation as well as IκBα... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Interleukin-6; Cyclic compressive force; P38 mitogen-activated protein kinases; Nuclear factor-κB; Osteocyte. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100437 |
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| Zhou,Huiqin; Du,Hong; Zhang,Haifang; Shen,Haiying; Yan,Ruhong; He,Yun; Wang,Min; Zhu,Xueming. |
| Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world's hot spots once again. The key issue of developing the vaccine of S. aureus is how to find an ideal key pathogenic gene of S. aureus. It was previously suggested that EsxA might be a very important factor in S. aureus abscess formation in mice, but clinical experimental evidence was lacking. We therefore expressed EsxA protein through prokaryotic expression system and purified EsxA protein by Ni-affinity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: S. aureus; EsxA; Anti-EsxA antibodies; Multi-drug resistant. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100038 |
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