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Henzel,Andréia; Brum,Mário Celso Sperotto; Lautert,Cláudia; Martins,Mathias; Lovato,Luciane Teresinha; Weiblen,Rudi. |
Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1) are the two primary causes of upper respiratory tract disease in cats. The aim of this study was to demonstrate the distribution of FCV and FHV-1 among the feline population of several counties in Rio Grande do Sul State, Brazil. To this end, conjunctival and nasal swabs were collected from 302 cats from different locations, including households, breeding catteries, veterinary clinics, animal hospitals and experimental research facilities. The samples were collected between July 2006 to June 2009. The virus isolation was performed in CRFK cells and, subsequently, the identification was confirmed by PCR. FCV, FHV-1, or both were isolated from 55 cats from 28 different locations. FCV alone was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: FCV; FHV-1; URTD; Epidemiology. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200017 |
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Vetter,Michele Regina; Staggemeier,Rodrigo; Vecchia,Andréia Dalla; Henzel,Andréia; Rigotto,Caroline; Spilki,Fernando Rosado. |
Human adenoviruses (HAdV), members of the Adenoviridae family, are excreted through the fecal route and may be present in the feces of humans consuming contaminated food or water. The presence of HAdV from different serotypes in the feces of healthy individuals was already reported using conventional polymerase chain reaction; however, real-time PCR (qPCR) may reveal not only the rates of detection as well as demonstrate the viral loads excreted by healthy persons. Aiming to identify and characterize the presence of adenoviruses in stool samples, 147 fecal samples from patients with no records of diarrhea were analyzed (74 from winter season and 73 from summer) by Real-Time PCR (qPCR) assay and conventional PCR. HAdV genome was present in 43.8% (32/73) of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HAdV; Stool samples; QPCR; Healthy individuals. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300749 |
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Luz,Roger B.; Staggemeier,Rodrigo; Fabres,Rafael B.; Soliman,Mayra C.; Souza,Fernanda G.; Gonçalves,Raoni; Fausto,Ivone V.; Rigotto,Caroline; Heinzelmann,Larissa S.; Henzel,Andréia; Fleck,Juliane D.; Spilki,Fernando R.. |
Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Farfantepenaeus paulensis; Pink-shrimp; Water quality; Northern Coast of Rio Grande do Sul. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300715 |
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Loguercio,Andrea Pinto; Battistin,Alice; Vargas,Agueda Castagna de; Henzel,Andréia; Witt,Niura Mazzini. |
A atividade antimicrobiana de plantas medicinais tem sido pesquisada em diversas espécies, tanto no Brasil quanto em outros países. O objetivo deste trabalho foi o de avaliar a existência de efeito antibacteriano de extrato hidro-alcoólico a 10% (m/v) de folhas de jambolão. Utilizaram-se 17 isolados bacterianos, Gram positivos e Gram negativos. A ação antibacteriana foi avaliada através da inoculação de placas de ágar Mueller Hinton, com um inóculo bacteriano de 1x10(6) ufc mL-1, onde se colocaram quatro discos de papel; o primeiro de antimicrobiano comercial e os demais embebidos em 25mL do extrato, de solução salina ou de etanol. As placas foram incubadas a 37°C por 24 horas, sendo posteriormente realizada a leitura do diâmetro dos halos de inibição. O... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Planta medicinal; Antimicrobiano; Jambolão. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000200019 |
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