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	Provedor de dados 116 (1) 65 (1) Autor Herculano,Polyanna Nunes (2) Moreira,Keila Aparecida (2) Silva,Anna Carolina da (2) Gomes,José Erick Galindo (1) Nascimento,Talita Camila Evaristo da Silva (1) Queiroz,Alana Emilia Soares de França (1) Silva,Sheylla Araújo da (1) Soares,Elisângela França (1) Mais... Palavra-chave Atividade proteolítica (1) Coagulante vegetal (1) Enzimas coagulantes do leite (1) Fruta-pão (1) Plant proteases (1) Proteolytic activity (1) Rennet (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2013 (1) País Brazil (2) Idioma Inglês (1) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent 116 Silva,Anna Carolina da; Nascimento,Talita Camila Evaristo da Silva; Silva,Sheylla Araújo da; Herculano,Polyanna Nunes; Moreira,Keila Aparecida. There are several obstacles to the use of chymosin in cheese production. Consequently, plant proteases have been studied as possible rennet substitutes, but most of these enzymes are unsuitable for the manufacture of cheese. The aim of this study was to evaluate the potential of latex from Sideroxylon obtusifolium as a source of milk-clotting proteases and to partially characterize the enzyme. The enzyme extract showed high protease and coagulant activities, with an optimal pH of 8.0 and temperature of 55 °C. The enzyme was stable in wide ranges of temperature and pH. Its activity was not affected by any metal ions tested; but was inhibited by phenylmethanesulfonyl fluoride and pepstatin. For the coagulant activity, the optimal concentration of CaCl2 was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteolytic activity; Plant proteases; Rennet. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300017 Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite 65 Soares,Elisângela França; Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Gomes,José Erick Galindo; Herculano,Polyanna Nunes; Moreira,Keila Aparecida. Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzimas coagulantes do leite; Atividade proteolítica; Coagulante vegetal; Fruta-pão. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco