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Polymorphism C373G of the PECAM-1 Gene in Chilean Subjects with Coronary Disease and Controls International Journal of Morphology
Herrera,Christian L; Jaramillo,Priscilla C; Lanas,Fernando; Salazar,Luis A.
The platelet endothelial cell adhesion molecule-1 (PECAM-1), a 130-kDa membrane glycoprotein, is expressed on the surface of monocytes, some T-lymphocyte subsets, neutrophils, platelets and endothelial cells. PECAM-1 plays a key role in the transendothelial migration of circulating leukocytes during vascular inflammation. The aim of the present investigation was to evaluate the association between the C373G polymorphism of the PECAM-1 gene and coronary artery disease in Chilean subjects. A total of 220 individuals were investigated (112 cases and 108 controls). The presence of coronary artery disease was confirmed by angiography (Stenosis >70%). The C373G polymorphism was detected by polymerase chain reaction followed enzymatic restriction. The genotype...
Tipo: Journal article Palavras-chave: Aterosclerosis; PECAM-1; Polymorphism.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200031
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Detección Molecular de Estreptococos Cariogénicos en Saliva International Journal of Morphology
Salazar,Luis A; Vásquez,Claudio; Almuna,Alejandro; Oporto,Gonzalo; Santana,Roberto; Herrera,Christian L; Sanhueza,Antonio.
Streptococcus mutans y Streptococcus sobrinus han sido indicados como los principales agentes etiológicos de la caries dental. Sin embargo, los métodos microbiológicos y bioquímicos, disponibles actualmente en Chile, no permiten la rápida detección e identificación de estas bacterias. El objetivo de este trabajo fue implementar la metodología de reacción en cadena de la polimerasa (PCR) para detectar la presencia de S. mutans y S. sobrinus en saliva. Participaron de este estudio 51 escolares (5 a 17 años), provenientes de cinco diferentes colegios de la ciudad de Temuco; a los cuales se les realizó recuento de estreptococos del grupo mutans en saliva por método microbiológico y la diferenciación de especies por la técnica de PCR. Los resultados mostraron...
Tipo: Journal article Palavras-chave: Caries; Streptococcus mutans; PCR.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400027
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El Propóleos Reduce la Esteatosis Hepática Inducida por Dieta en Ratones International Journal of Morphology
Herrera,Christian L; Fritz,Osvaldo; Montenegro,Gloria; Alvear,Marysol; del Sol,Mariano; Salazar,Luis A.
El hígado graso no alcohólico (HGNA), comprende un amplio espectro de lesiones, que van desde esteatosis hepática, hasta cirrosis y carcinoma hepatocelular, siendo sus principales factores de riesgo los desordenes asociados a síndrome metabólico (SM). El propóleos, sustancias resinosa elaborada por Apis mellifera para la protección de la colmena, ha demostrado un efecto hepatoprotector, así el objetivo de esta investigación fue evaluar el efecto de un propóleos chileno sobre el desarrollo de esteatosis hepática no alcohólica en ratones C57BL/6J sometidos a una dieta aterogénica tipo Paigen. Veintiocho ratones (C57BL/6J), divididos en: 1, dieta balanceada (CH); 2, dieta hipercolesterolémica (HC); 3, dieta HC + 10 mg/kg/día de propóleos (BP); 4, dieta HC +...
Tipo: Journal article Palavras-chave: Esteatosis hepática; HGNA; Dieta aterogénica; Propóleos.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100010
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The antifungal effect of six commercial extracts of Chilean propolis on Candida spp Ciencia e Investigación Agraria
Herrera,Christian L; Alvear,Marysol; Barrientos,Leticia; Montenegro,Gloria; Salazar,Luis A.
Propolis has been used in traditional medicine for many centuries because of its benefcial health properties, including its antimicrobial capacity. Prosthesis stomatitis affects a signifcant percentage of users of removable dentures; Candida albicans is the most common fungal species associated with the development of this pathology. Thus, the objectives of this study were: a. To evaluate the antifungal activity of six commercial propolis extracts against Candida spp. that was isolated from the oral cavity of removable dentures users, and b. To determine chemical characteristics of the propolis extracts evaluated. Among the results, we note that these concentrations of polyphenols varied between 9 ± 0.3 and 85 ± 2.1 mg mL-1. Chromatographic analysis was...
Tipo: Journal article Palavras-chave: Antifungal activity; Candida albicans; Propolis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000100007
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Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum Ciencia e Investigación Agraria
Saavedra,Nicolás; Barrientos,Leticia; Herrera,Christian L; Alvear,Marysol; Montenegro,Gloria; Salazar,Luis A.
Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after...
Tipo: Journal article Palavras-chave: Lactobacillus fermentum; Dental caries; Propolis; Antibacterial activity.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011
Registros recuperados: 5
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