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| Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto. |
| This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as... |
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Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154 |
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