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| Higuti,Ilma Hiroko; Silva,Priscila Anunciação da; Papp,Juliana; Okiyama,Vivian Mayumi de Eiróz; Andrade,Edicléia Alves de; Marcondes,Aluizio de Abreu; Nascimento,Aguinaldo José do. |
| Cyclodextrin glycosyltransferase (EC 2.4.1.19, CGTase) production from B. firmus, isolated from soil of Curitiba, PR, was optimized in shake flask using an experimental design approach. The CGTase was produced when the carbon source was starch and beta-CD, but when simple sugars such as glucose, galactose, lactose, sucrose, and maltose were used, there was no enzyme production. CGTase production was the same with either organic nitrogen or inorganic nitrogen source. CGTase activity decreased 2-fold when incubation temperature was increased from 28 to 37 ° C, and decreased 2.1- fold when the initial pH was lowered from 10.3 to 7.4. The colorimetric determinations of alpha - and beta -CD were analyzed as a non-linear relationship and the equilibrium constant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclodextrin glycosyltransferase; Bacillus firmus; Cyclodextrins. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600001 |
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| Higuti,Ilma Hiroko; Macena,Ivone Rodrigues; Masunari,Setuko; Branco Filho,Mario de Oliveira; Blaskowiski,Marita Maciel Moreira; Nascimento,Aguinaldo José do. |
| The Perequê and Penedo rivers flow through the plains of Praia de Leste and fall into the sea between the municipalities of Pontal do Sul and Ponta do Poço. Both of these rivers belong to the sub-basin of Paranaguá Bay, and are constituent parts of the hydrographic basin of the Atlantic Ocean (State of Paraná). Four sites along the Perequê River (Stations 1-4) and one along the Penedo River (Station 5) were monitorated for water pollution over a 12-month period. During the period of December 1995 to November 1996, three water samples were collected from each site, placed in sterilized bottles and transported to the laboratory in ice-boxes. They were analyzed for the occurrence of total and fecal coliforms, temperature and pH. Fecal pollution was detected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Total coliforms; Fecal coliforms; Water microbiology. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000400005 |
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| Nogueira,Keite da Silva; Higuti,Ilma Hiroko; Nascimento,Agnaldo José do; Terasawa,Larissa Bail; Oliveira,Simone de; Matos,Adriana Pereira; Souza,Helena Aguilar Peres Homem de Mello de; Cogo,Laura Lúcia; Dalla Costa,Libera Maria. |
| Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESBL; Beta-lactamases; Enterobacteriaceae. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006 |
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