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Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure BJM
Ferreira,Fernanda Stoduto; Horvath,Mariana Bandeira; Tondo,Eduardo Cesar.
The objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella Enteritidis SE86; Diluents; Plating methods; Stressed cells; Sodium dichloroi-socyanurate.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300018
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Occurrence of Salmonella spp. and generic Escherichia coli on beef carcasses sampled at a brazilian slaughterhouse BJM
Silva,Fabiana Fernanda Pacheco da; Horvath,Mariana Bandeira; Silveira,Juliana Guedes; Pieta,Luiza; Tondo,Eduardo Cesar.
A total of 120 beef carcasses were analyzed during processing at a slaughterhouse in southern Brazil. The carcasses were sampled by swab at three different steps of the slaughter line and then they were tested for Salmonella and E. coli. The Salmonella isolates were also examined for antimicrobial susceptibility. Salmonella prevalence distribution was modeled and the probability of contamination was simulated using @Risk program and 10,000 interactions. Results demonstrated that 4 beef carcasses (3.3%) were positive for Salmonella only in the first point. The six isolates of Salmonella were classified: S. Newport (n = 3), S. Saintpaul (n = 2) and S. Anatum (n = 1). No Salmonella strains exhibited resistance to any of the antimicrobials tested. As expected,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; E. coli; Beef carcasses; Slaughter line; Antimicrobial susceptibility.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100004
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