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	Provedor de dados BJMBR (1) BJM (1) Autor Hou,Lin (2) Cheng,Fei (1) Ge,Yinlin (1) Peng,Xiaobang (1) Shang,Zhengchun (1) Wang,Qing (1) Woeste,Keith (1) Xue,Meilan (1) Zhang,Jinyu (1) Zhang,Shuoxin (1) Zhao,Peng (1) Mais... Palavra-chave Breast cancer cells (1) Cell lysis (1) Chlorella vulgaris (1) CvFad3 gene (1) DNA extraction method (1) DNA purity (1) Fatty acid composition (1) Fatty acid desaturase (1) Fungal community (1) Recombinant expression vector (1) Terminal restriction fragment length polymorphism (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 2012 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells BJMBR Xue,Meilan; Ge,Yinlin; Zhang,Jinyu; Wang,Qing; Hou,Lin. Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorella vulgaris; CvFad3 gene; Fatty acid desaturase; Recombinant expression vector; Fatty acid composition; Breast cancer cells. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200006 Soil pretreatment and fast cell lysis for direct polymerase chain reaction from forest soils for terminal restriction fragment length polymorphism analysis of fungal communities BJM Cheng,Fei; Hou,Lin; Woeste,Keith; Shang,Zhengchun; Peng,Xiaobang; Zhao,Peng; Zhang,Shuoxin. Abstract Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, five prewashing agents were compared in terms of their efficiency and effectiveness in removing soil contaminants. Residual contaminants were precipitated by adding 0.6 mL of 0.5 M CaCl2. Four cell lysis methods were applied to test their compatibility with the pretreatment (prewashing + Ca2+ flocculation) and to ultimately identify the optimal cell... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell lysis; DNA extraction method; DNA purity; Terminal restriction fragment length polymorphism; Fungal community. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400817 Registros recuperados: 2 Primeira ... 1 ... Última

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