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Report on the technical and analytical improvements of innovative techniques and recommendations on their use ArchiMer
Artigas, Felipe; Creach, Veronique; Houliez, Emilie; Karlson, Bengt; Lizon, Fabrice; Seppala, Jukka; Wacquet, Guillaume.
This is a summary of the activities and results of JERICO-NEXT Work Package 3 Innovations in Technology and Methodology, Task 3.1 Automated platform for the observation of phytoplankton diversity in relation to ecosystem services. The aim is to provide an advanced report on the last developments dedicated to the observation of the phytoplankton diversity by applying novel techniques on automated platforms. The work was carried out in close connection with task 4.1 Biodiversity of plankton, harmful algal blooms and eutrophication. The partners involved in these developments are CNRS, SYKE, SMHI, HZG, RWS, VLIZ CEFAS and Ifremer. Subcontractors in WP4, task 4.1 are WHOI, Scanfjord AB, Tomas Rutten b.v., CytoBuoy b.v. and UGent - PAE. The work was carried out...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00630/74242/73873.pdf
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Rapid light curves (RLC) or non-sequential steady-state light curves (N-SSLC): which fluorescence-based light response curve methodology robustly characterizes phytoplankton photosynthetic activity and acclimation status? ArchiMer
Houliez, Emilie; Lefebvre, Sebastien; Lizon, Fabrice; Schmitt, Francois G..
This study is the first in situ work comparing rapid light curves (RLC) and non-sequential steady-state light curves (N-SSLC) in their efficiency to characterize phytoplankton photosynthetic activity and acclimation status. Measurements were carried out at two time scales (daily and annual) using the Pulse Amplitude Modulated fluorometry on samples taken in the coastal waters of a macrotidal ecosystem (the Strait of Dover, eastern English Channel). RLC and N-SSLC were compared under a wide range of environmental conditions and phytoplankton composition in order to define the best methodology to accurately capture short and long-term adjustments in the functioning of the photosynthetic apparatus. The relationships between the photosynthetic parameters...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00395/50596/51484.pdf
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Spectral fluorometric characterization of Haptophyte dynamics using the FluoroProbe: an application in the eastern English Channel for monitoring Phaeocystis globosa ArchiMer
Houliez, Emilie; Lizon, Fabrice; Thyssen, Melilotus; Artigas, Luis Felipe; Schmitt, Francois G..
In this study, we examined the possibility of using the FluoroProbe for monitoring the dynamics of the Haptophyte Phaeocystis globosa in the coastal waters of the eastern English Channel. The FluoroProbe was recalibrated by recording a new fingerprint for P. globosa and the use of this new fingerprint was tested through a series of laboratory and in situ experiments. The annual dynamics of P. globosa estimated using the FluoroProbe and by flow cytometry were similar. A strong relationship was found between the FluoroProbe estimates of P. globosa biomass expressed in terms of chlorophyll a equivalent per litre (eq. mu g L(-1)) and flow cytometric cell counts (r = 0.889, P < 0.001, n = 121). The FluoroProbe can be used to detect the flagellated cells as...
Tipo: Text Palavras-chave: Spectral fluorescence; Phaeocystis globosa; Haptophytes; Algal blooms; Phytoplankton quantification; Chlorophyll a.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00373/48461/48723.pdf
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