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| Martos,María Alicia; Zubreski,Emilce Roxana; Combina,Mariana; Garro,Oscar Alfredo; Hours,Roque Alberto. |
| The objective of the present study was the isolation of a yeast strain, from citrus fruit peels, able to produce a polygalacturonase by submerged fermentation with maceration activity of raw cassava roots. Among 160 yeast strains isolated from citrus peels, one strain exhibited the strongest pectinolytic activity. This yeast was identified as Wickerhamomyces anomalus by 5.8S-ITS RFLP analysis and confirmed by amplification of the nucleotide sequence. The yeast produced a polygalacturonase (PG) in Erlenmeyer shake flasks containing YNB, glucose, and citrus pectin. PG synthesis occurred during exponential growth phase, reaching 51 UE.mL-1 after 8 hours of fermentation. A growth yield (Yx/s) of 0.43 gram of cell dry weight per gram of glucose consumed was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Maceration; Cassava. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000200018 |
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| Gortari,María Cecilia; Hours,Roque Alberto. |
| Background: Chitin is an important natural resource. The annual worldwide production is estimated in approximately 10(10)-10(12) ton. It is produced by arthropods (insects and crustaceans), molluscs and fungi. Its main biological function is structural. Crustacean shells are the most important chitin source for commercial use due to its high content and ready availability. Chitin and its derivatives have great economical value because of their numerous applications: food, cosmetics, pharmaceuticals, textile industries, waste water treatment and agriculture. In nature, chitin is closely associated with proteins, minerals, lipid and pigments, which have to be removed. Results: Several techniques to extract chitin from different sources have been reported.... |
| Tipo: Journal article |
Palavras-chave: Biological extraction; Chitin production; Crustacean waste. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300014 |
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| Chesini,Mariana; Neila,Lorena Paola; de la Parra,Dante Fratebianchi; Rojas,Natalia Lorena; Contreras Esquivel,Juan Carlos; Cavalitto,Sebastian Fernando; Ghiringhelli,Pablo Daniel; Hours,Roque Alberto. |
| Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular... |
| Tipo: Journal article |
Palavras-chave: Acidophilic enzymes; Batch cultures; Inulin degradation; Submerged cultures. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300008 |
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