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	Provedor de dados BABT (1) BJM (1) Autor Iftikhar,Tehreema (2) Zia,Muhammad Anjum (2) Abbas,Rao Zahid (1) Ain,Qurat-ul (1) Haq,Ikram ul (1) Niaz,Mubashir (1) Rahman,Khalil-ur (1) Mais... Palavra-chave A. niger (1) Antibodies (1) Extracellular lipases (1) Fermentor (1) Glucose oxidase (1) Kinetics (1) Rabbits (1) Rhizopus oligosporus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Production of rabbit antibodies against purified Glucose oxidase BABT Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur. Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008 Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor BJM Iftikhar,Tehreema; Niaz,Mubashir; Zia,Muhammad Anjum; Haq,Ikram ul. The present investigation deals with the kinetics of submerged extracellular lipases fermentation by both wild and mutant strains of Rhizopus oligosporus var. microsporus in a laboratory scale stirred fermentor. Other parameters studied were inoculum size, pH, agitation and rate of aeration. It was found that the growth and lipases production was increased gradually and reached its maximum 9.07± 0.42ª U mL-1 (W) and 42.49 ± 3.91ª U mL-1 (M) after 30h of fermentation for both wild and mutant strain. There is overall increase of 109% (W) and 124% (M) in the production of extracellular lipases as compared to shake flask. Another significant finding of the present study is that the fermentation period is reduced to 30 h in case of wild and 23 h in case of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipases; Rhizopus oligosporus; Fermentor; Kinetics. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400034 Registros recuperados: 2 Primeira ... 1 ... Última

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