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Fatal case of bacteremia caused by an atypical strain of Corynebacterium mucifaciens BJID
Cantarelli,Vlademir Vicente; Brodt,Teresa Cristina. Z.; Secchi,Carina; Inamine,Everton; Pereira,Fabiana de Souza; Pilger,Diogo Andre.
Corynebacterium species have often been considered normal skin flora or contaminants; however, in recent years they have been increasingly implicated in serious infections. Moreover, many new species have been discovered and old species renamed, especially after molecular biology techniques were introduced. Corynebacterium mucifaciens is mainly isolated from blood and from other normally-sterile body fluids; it forms slightly yellow, mucoid colonies on blood agar. We report a fatal case of bacteremia due to an atypical strain of C. mucifaciens. This strain had atypical colony morphology; analysis of the 16S rRNA gene was used to define the species.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bacteremia; Blood infection; Corynebacterium; Corynebacterium mucifaciens.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600013
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Utility of the ceftazidime-imipenem antagonism test (CIAT) to detect and confirm the presence of inducible AmpC beta-lactamases among enterobacteriaceae BJID
Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Z.; Secchi,Carina; Cavalcante,Bianca C.; Pereira,Fabiana de Souza.
Detection of AmpC beta-lactamase production by enterobacteria has been problematic. Contrary to ESBLs, no specific guidelines are available for detection and confirmation of AmpC production by clinical relevant microorganisms. Moreover, some bacterial species may produce inducible AmpC beta-lactamases that can be easily overlooked by routine susceptibility tests. We reported here a new test based on the strong inducible effect of imipenem on AmpC genes and the consequent antagonism with ceftazidime. This test is very simple and proved to be helpful in detecting AmpC-inducible enzymes among several species of clinical isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: AmpC; Inductible beta-lactamases; Screening tests; Imipenem; Cefoxitin screening.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200014
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Quality control for microbiological culture media: Is it enough to follow the NCCLS M22-A2 procedures? BJM
Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza; Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas.
Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quality control; Culture medium; NCCLS.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003
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