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In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo International Journal of Morphology
Intarapat,Sittipon; Gonmanee,Thanasup; Thonabulsombat,Charoensri.
The aim of this study was to observe morphological changes of the cultured otocysts isolated from various stages of the chick embryo. Isolated otocysts were dissected from embryonic day, E2.5-4.5 of incubation (HH stage 16-26) according to stages of developing inner ear. Morphology of the chick otocyst exhibited an ovoid shape. The width and height of the otocyst were 0.2 mm and 0.3 mm, respectively. Elongation of a tube-like structure, the endolymphatic duct, was found at the dorsal aspect of the otocyst. The cultured otocyst is lined by the otic epithelium and surrounding periotic mesenchymal cells started to migrate outwards the lateral aspect of such epithelium. Notably, the acoustic-vestibular ganglion (AVG) was observed at the ventrolateral aspect of...
Tipo: Journal article Palavras-chave: Chick embryo; Inner ear; Otocyst; Otic development.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100034
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Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation International Journal of Morphology
Intarapat,Sittipon; Sailasuta,Achariya; Satayalai,Orawan.
In birds, male embryo the gonads develop bilateral testes, in which both left and right sides produce functional spermatozoa, whereas female embryo, only the left gonad develops into a functional ovary. Estrogen plays a key role in avian sex determination in both sexes by binding to the estrogen receptor (ER). Surprisingly, chicken estrogen receptor (cER) mRNA is expressed in both sexes; moreover; its expression is only expressed in the left male gonad. The present study aimed to localize ER protein in the left gonad of male quail embryo using immunohistochemistry. The 8-day-old male quail embryos whose embryonic sex distinguished by gonadal morphology were studied. Histology of the left male gonad displayed thin cortex containing 1 to 2 layers of the...
Tipo: Journal article Palavras-chave: Estrogen receptor; Embryonic gonad; Gonadal differentiation; Quail embryo.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200026
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