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	Provedor de dados BJM (2) BABT (1) Autor Irfan,Muhammad (3) Nadeem,Muhammad (2) Abduljabbar, (1) Ahmed,Sajjad (1) Ali,Sajid (1) Khan,Anwar Sheed (1) Khan,Muhammad Tahir (1) Khattak,Yasir (1) Mushtaq,Zareena (1) Naz,Mammona (1) Sajjad,Wasim (1) Syed,Quratualain (1) Syed,Quratulain (1) Mais... Palavra-chave Characterization (1) Detergent stability (1) Ethanol production (1) GeneXpert (1) Kinetics (1) LED-FM microscopy (1) Lignoccellulosic biomass (1) Pretreatment (1) Protease (1) Purification (1) Rhizopus oryzae (1) Sacchromyces cervisae (1) Sensitivity (1) Specificity (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (1) 2015 (1) 2014 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae BABT Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain. In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175 Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan BJM Khan,Anwar Sheed; Ali,Sajid; Khan,Muhammad Tahir; Ahmed,Sajjad; Khattak,Yasir; Abduljabbar,; Irfan,Muhammad; Sajjad,Wasim. ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as... Tipo: Info:eu-repo/semantics/article Palavras-chave: GeneXpert; LED-FM microscopy; Sensitivity; Specificity. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400909 Ethanol production from agricultural wastes using Sacchromyces cervisae BJM Irfan,Muhammad; Nadeem,Muhammad; Syed,Quratualain. The main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by Sacchromyces cervisae. All the three substrates were ground to powder form (2 mm) and pretreated with 3%H2O2 + 2% NaOH followed by steaming at 130 °C for 60 min. These substrates were hydrolyzed by commercial cellulase enzyme. The whole fermentation process was carried out in 500 mL Erlenmeyer flask under anaerobic conditions in submerged fermentation at 30 °C for three days of incubation period. FTIR analysis of the substrates indicated significant changes in the alteration of the structure occurred after pretreatment which leads to efficient saccharification. After pretreatment the substrates were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignoccellulosic biomass; Pretreatment; Sacchromyces cervisae; Ethanol production. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200012 Registros recuperados: 3 Primeira ... 1 ... Última

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