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Isolation of rabies virus from the parotid salivary glands of foxes (Pseudalopex vetulus) from Paraíba State, Northeastern Brazil BJM
Silva,Maria Luana Cristiny Rodrigues; Lima,Fabiano da Silva; Gomes,Albério Antônio de Barros; Azevedo,Sérgio Santos de; Alves,Clebert José; Bernardi,Fernanda; Ito,Fumio Honma.
To determine the presence of rabies virus in the parotid salivary glands, 12 road-killed rabies-positive hoary foxes (Pseudoalopex vetulus) were tested by using the fluorescent antibody test (FAT) and mouse inoculation test (MIT). All 12 parotid salivary glands were positive for both tests, although in some cases several passages were required. The findings of this study support the importance of the hoary fox as rabies reservoir in the semi-arid region of Paraíba State, Northeastern Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parotid gland; Fox; Rabies virus; Isolation; Paraíba State.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300004
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Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells BJM
Jerez,José Antonio; Gregori,Fábio; Brandão,Paulo Eduardo; Rodriguez,Cesar Alejandro Rosales; Ito,Fumio Honma; Buzinaro,Maria da Glória; Sakai,Takeo.
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine coronavirus (BCoV); Isolation; HmLu-1 cells.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001
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Cross neutralizing antibodies in hamsters vaccinated with leptospiral bacterins produced with three serovars of serogroup Sejroe BJM
Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda.
Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016
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Paralytic rabies in swine BJM
Pessoa,Clarice Ricardo de Macedo; Silva,Maria Luana Cristiny Rodrigues; Gomes,Albério Antônio de Barros; Garcia,Andrea Isabel Estévez; Ito,Fumio Honma; Brandão,Paulo Eduardo; Riet-Correa,Franklin.
Rabies transmitted by vampire bats was diagnosed in pigs with paralysis of the pelvic limbs. Diffuse nonsuppurative encephalomyelitis, affecting mainly the spinal cord, was observed histologically. Despite the various diagnosis of rabies in pigs this is the first report of clinical signs and pathology of rabies transmitted by vampire bats.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vampire bats; Epidemiology; Virus isolation; Zoonotic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100038
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