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	Provedor de dados Trop. Plant Pathol. (1) Autor FAJARDO,THOR V.M. (1) JELKMANN,WILHELM (1) KUHN,GILMAR B. (1) NICKEL,OSMAR (1) Palavra-chave Capillovirus (1) Molecular characterization (1) RT-PCR (1) Tipo do documento Info:eu-repo/semantics/other (1) Ano 2001 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil Trop. Plant Pathol. NICKEL,OSMAR; FAJARDO,THOR V.M.; JELKMANN,WILHELM; KUHN,GILMAR B.. Apple stem grooving virus (ASGV) is one of the most important viruses infecting fruit trees. This study aimed at the molecular characterization of ASGV infecting apple (Malus domestica) plants in Santa Catarina (SC). RNA extracted from plants infected with isolate UV01 was used as a template for RT-PCR using specific primers. An amplified DNA fragment of 755 bp was sequenced. The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted Mr of approximately 27 kDa. The nucleotide and the deduced amino acid sequences of the coat protein gene showed identities of 90.9% and 97.9%, respectively, with a Japanese isolate of ASGV. Very high amino acid homologies (98.7%) were also found with Citrus... Tipo: Info:eu-repo/semantics/other Palavras-chave: Molecular characterization; Capillovirus; RT-PCR. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300014 Registros recuperados: 1 Primeira ... 1 ... Última

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