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| Thonar, C.; Erb, A.; Jansa, J.. |
| Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific motifs in the nuclear large ribosomal subunit RNA genes. Design of such markers is complicated by the multinuclear and multigenomic cellular organization of these fungi and the high DNA sequence diversity within the smallest biologically relevant units (i.e. single-spore isolates). These limitations are further compounded by inefficient biomass production of these fungi, resulting in limited availability of pure genomic DNA (gDNA) of... |
| Tipo: Journal paper |
Palavras-chave: Indicators and other value-laden measures; Soil. |
| Ano: 2012 |
URL: http://orgprints.org/21817/1/thonar-etal-2012-MolecularEcol-Resources-Vol12-p219-232.pdf |
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| Thonar, C.; Frossard, E.; Šmilauer, P.; Jansa, J.. |
| Interactions between arbuscular mycorrhizal fungal (AMF) species cocolonizing the same host plant are still little understood in spite of major ecological significance of mycorrhizal symbiosis and widespread occurrence of these fungi in communities rather than alone. Furthermore, shifting the composition of AMF communities has demonstrated consequences for the provision of symbiotic benefits to the host as well as for the qualities of ecosystem services. Therefore, here we addressed the nature and strength of interactions between three different AMF species in all possible two-species combinations on a gradient of inoculation densities. Fungal communities were established in pots with Medicago truncatula plants, and their composition was assessed with... |
| Tipo: Journal paper |
Palavras-chave: Soil. |
| Ano: 2014 |
URL: http://orgprints.org/26019/1/Thonar_etal_2014_ArbMycorrFungi.pdf |
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| Messmer, M.; Berner, A.; Krauss, M.; Jansa, J.; Presterl, Th.; Schmidt, W.; Mäder, P.. |
| Conservation tillage (no-till and reduced tillage) brings many benefits with respect to soil fertility and energy use, but it also has drawbacks regarding the need for synthetic fertilizers and herbicides. To promote conversation tillage in organic farming systems, crop rotation, fertilization and weed control have to be optimized. In addition, crop varieties are needed with improved nutrient use efficiency (NUE) and high weed competitiveness or tolerance. |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Soil quality; Soil tillage; Weed management. |
| Ano: 2010 |
URL: http://orgprints.org/18931/1/Messmer-etal-2011-NUE-AMF-Eucarpia-printed.pdf |
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| Lendenmann, M.; Thonar, C.; Barnard, R.L.; Salmon, Y.; Werner, R.A.; Frossard, E.; Jansa, J.. |
| Many studies have scrutinized the nutritional benefits of arbuscular mycorrhizal associations to their host plants, while the carbon (C) balance of the symbiosis has often been neglected. Here, we present quantification of both the C costs and the phosphorus (P) uptake benefits of mycorrhizal association between barrel medic (Medicago truncatula) and three arbuscular mycorrhizal fungal species, namely Glomus intraradices, Glomus claroideum, and Gigaspora margarita. Plant growth, P uptake and C allocation were assessed 7 weeks after sowing by comparing inoculated plants with their non-mycorrhizal counterparts, supplemented with different amounts of P. Isotope tracing (3)(3)P and (1)(3)C) was used to quantify both the mycorrhizal benefits and the costs,... |
| Tipo: Journal paper |
Palavras-chave: Soil. |
| Ano: 2011 |
URL: http://orgprints.org/26367/1/Lendenmann_etal_2011_mycorrhiza.pdf |
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| Thonar, Cécile; Erb, A.; Jansa, J.. |
| Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific motifs in the nuclear large ribosomal subunit RNA genes. Design of such markers is complicated by the multinuclear and multigenomic cellular organization of these fungi and the high DNA sequence diversity within the smallest biologically relevant units (i.e. single-spore isolates). These limitations are further compounded by inefficient biomass production of these fungi, resulting in limited availability of pure genomic DNA (gDNA) of... |
| Tipo: Journal paper |
Palavras-chave: Soil. |
| Ano: 2011 |
URL: http://orgprints.org/25988/1/Thonar_et_al_2011_CT33.pdf |
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