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Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance BABT
Deeba,Farah; Sultana,Tasawar; Javaid,Bushra; Mahmood,Tariq; Naqvi,S. M. S..
ABSTRACT The MYB family represents one of the most abundant classes of transcriptional regulators that perform pivotal role under different developmental processes and abiotic stresses. In present study, a MYB gene from Oryza sativa was selected for functional characterization. Bioinformatics analysis revealed that OsMYB1 cDNA encodes R2-R3 type DNA binding domain consisting of 413 amino acids having size of 44 kDa and pI of 6.24. DNA binding domain containing region was cloned and over-expressed in E. coli. Then, the survival of pGEX-OsMYB1 transformed E. coli cells was compared with control plasmid under different concentrations of NaCl, mannitol, high and low temperature. pGEX-OsMYB1 enhanced the survival of cells at high temperature and salinity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; E. coli; Oryza sativa; OsMYB1; PGEX; Real time qPCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100411
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