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| Shi,Weifeng; Li,Kun; Ji,Yun; Jiang,Qinbo; Wang,Yuyue; Shi,Mei; Mi,Zuhuang. |
| Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin) genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR). The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the resistant isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella pneumoniae; AmpC β-Lactamase; Porin; Molecular biology. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200015 |
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| Shi,Mei; Zhou,Yaping; Cao,Limin; Ding,Cuijun; Ji,Yun; Jiang,Qinbo; Liu,Xiping; Li,Xiang; Hou,Xueling; Peng,Hongjun; Shi,Weifeng. |
| The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterovirus 71; Gene cloning; Recombinant VPl protein; ELISA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400027 |
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