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	Provedor de dados Nature Precedings (2) Autor Jianxing Song (2) Haina Qin (1) Linlin Miao (1) Linlin Miao (1) Palavra-chave Chemistry (2) Evolutionary Biology (2) Molecular Cell Biology (2) Earth & Environment (1) Tipo do documento Manuscript (2) Ano 2012 (2) País United Kingdom (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Solubilization of M2 Transmembrane Peptide of Influenza A in Pure Water: Implications for Emergence of Proteins and Protein-embedded Primeval Membranes in Unsalted Oceans Nature Precedings Jianxing Song; Linlin Miao. We demonstrated that M2 transmembrane peptide, one of the most hydrophobic sequences in nature, can be solublized to at least ~100 µM in unsalted water without any lipid molecules. Strikingly, the M2 peptide also forms a highly-helical conformation in water which remains almost unchanged even at 95 ºC, as characterized by CD spectroscopy. Our result has critical implications in understanding emergence of proteins and protein-embedded primeval membranes in unsalted oceans. Tipo: Manuscript Palavras-chave: Chemistry; Molecular Cell Biology; Evolutionary Biology. Ano: 2012 URL: http://precedings.nature.com/documents/6773/version/1 "Dark Mediators" of Proteins as Revealed by NMR in Water: Residue-selective Anion Bindings that are Masked by Pre-existing Buffer Nature Precedings Jianxing Song; Linlin Miao; Haina Qin. Ions are commonly believed to impose their effects on proteins by unspecific electrostatic screening. Here, by NMR we reveal that in water sulfate, chloride and thiocyanate are able to bind a well-folded WW domain at distinctive residues and affinities, which is surprisingly masked by the pre-existing buffer. Our study reveals that the specific anion binding is so ubiquitous and consequently no longer negligible in establishing "postreductionist framework" for protein biochemistry. Tipo: Manuscript Palavras-chave: Chemistry; Molecular Cell Biology; Earth & Environment; Evolutionary Biology. Ano: 2012 URL: http://precedings.nature.com/documents/6769/version/1 Registros recuperados: 2 Primeira ... 1 ... Última

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