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| Registros recuperados: 76 | |
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| Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence. |
| Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in... |
| Tipo: Text |
Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf |
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| Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Goubet, Anne; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Costantini, Louis; Masson, Jean-claude; Martin, Anne-genevieve. |
| Created in 1986, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91 /67/EEC and 95/70/EC and are a patt of the institutional tasks ofIfremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to survey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Banc de Granville and zone X). In 2004, no sample couId be analysed to these two areas because of the difficulty to obtain flat oysters (sparse oyster beds,... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00086/19702/17343.pdf |
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| Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique. |
| Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,... |
| Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf |
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| Kopp, Joel; Joly, Jean-pierre; Legagneur, Eric; Ruelle, Francois. |
| L'étude des stocks ostréicoles et mytilicoles a été réalisée pour la première fois sur l'ensemble des bassins conchylicoles de Basse-Normandie en 1995, sauf sur le secteur mytilicole d'Utah Beach (côte Est du Cotentin) où l'étude n'a pu être faite qu'en 1996. A partir des stratégies d'échantillonnages établies lors des études précédentes, les stocks en élevage ont été estimés à 71.728 tonnes (dont 44.051 t commercialisables) pour les huîtres en octobre, et 13.289 tonnes nettes pour les moules en mai. Si le stock de moules apparaît stable depuis la dernière estimation de 1993, on observe une importante augmentation de la biomasse d'huîtres depuis 1991. Cette augmentation est rattachée à une meilleure exploitation de secteurs peu exploités précédemment, la... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Mytiliculture; Estimation des stocks; Croissance; Normandie. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00092/20368/18030.pdf |
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| Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle. |
| Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using... |
| Tipo: Text |
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf |
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| Garcia, Celine; Haond, Christophe; Chollet, Bruno; Nerac, Mirella; Omnes, Emmanuelle; Joly, Jean-pierre; Dubreuil, Christine; Serpin, Delphine; Langlade, Aime; Le Gal, Dominique; Terre-terrillon, Aouregan; Courtois, Olivier; Guichard, Benjamin; Arzul, Isabelle. |
| Background: Microcell parasites are small intracellular protozoans mostly detected in molluscs and can be associated with mortalities. In 2010 and 2011, strong increases in mortality events were reported in different wild beds of the wedge clam Donax trunculus Linnaeus, along the Atlantic coast of France and the presence of potential pathogens, including microcells, was investigated. Methods: Clams collected in different beds showing mortality were examined by histology. Based on histological observations, confirmatory analyses were carried out, including transmission electron microscopy (TEM) and molecular characterization. Results: Histological analyses revealed the presence of small protozoans similar to microcell parasites in different tissues of Donax... |
| Tipo: Text |
Palavras-chave: Mikrocytos veneroides n. sp.; Mikrocytos donaxi n. sp.; Donax trunculus; Mortality; Microcell parasite. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/53989/55149.pdf |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf |
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| Kopp, Joel; Joly, Jean-pierre; Le Gagneur, Eric; Ruelle, Francois. |
| Grâce à une approche multidisciplinaire, ce document constitue la synthèse des connaissances acquises sur la filière conchylicole (huîtres creuses et moules) de la côte Ouest du Cotentin depuis 3 ans. Les aspects positifs, les contraintes et les difficultés physiques, administratives, socio-économiques et biologiques qui caractérisent cette activité ont été abordés tour à tour. Leur synergie a été analysée pour mettre en évidence les mesures de gestion réaliste les plus adaptées. |
| Tipo: Text |
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| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00082/19323/16917.pdf |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie. |
| La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en... |
| Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf |
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| Michel, C.; Bernardet, J. -f.; Castric, J.; Joly, Jean-pierre. |
| A great variety of pathogenic organisms present in the water may affect the vertebrate and invertebrate species cultured in different aquatic habitats. Compared to humans and terrestrial species, the diagnostic of aquatic animal diseases has long been hampered by scarce taxonomic knowledge about the relevant pathogens, but advances resulting from genetic and molecular developments has now resulted in a complete panoply of suitable techniques for this purpose. Choosing a diagnostic method should be done according to clinical, sanitary or officially prescribed considerations, and also according to the purpose of the procedure, should it be the correct identification or the detection of a pathogen. A critical overview of the techniques available of common use... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-6133.pdf |
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| Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva. |
| At the present time losses due to diseases are one of main factors limiting the aquaculture development. Unceasing increase of exchanges between different countries favours the risk of introduction and propagation of new pathogen agents. At the beginning of the 90’s the European Union laid the surveillance of mollusc health inside each Member State in order to protect the safety of exchanges. Indeed few measures are available to protect molluscs from diseases. Only the prophylactic approach is possible; this approach is based on a zoosanitary surveillance of mollusc populations and/or a development of resistant populations against some diseases. Created in 1986, the French network for surveillance and monitoring of mollusc health (REPAMO) ensures the... |
| Tipo: Text |
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| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00442/55334/56826.pdf |
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| Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Ravel, Christophe. |
| Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de deux Directives Européennes, les Directives 91 /67/CEE et 95/70/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae et la Marteiliose à Marteilia refringens chez l'huître... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Mollusques; Coquillages; Santé. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00086/19708/17349.pdf |
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| Goulletquer, Philippe; Joly, Jean-pierre; Gerard, Andre; Le Gagneur, Eric; Moriceau, Jacques; Peignon, Jean-marie; Heurtebise, Serge; Phelipot, Pascal. |
| Triploid oysters Crassllstrea gigas were: produced in 1990 by treating fertilized eggs with cytochalasin B (CS). Triploids, treated diploids, and controls were deployed early 1991 in a high carrying capacity ecosystem on the Eastern Coast of Normandy (France). A monthly monitoring in 1992 showed that triploids yielded significantly higher growth rate and biochemical composition. However, growth was more heterogeneous. No evidence was found for a length or weight bimodal frequency distribution within triploid groups after a 26 months rearing cycle. Triploids showed retardation of gametogenesis. Carbohydrates content in triploids remained almost constant (40%) from June to September. Their survival rates were significantly lower than controls. Moreover... |
| Tipo: Text |
Palavras-chave: Biochemical compostion; Survival; Carrying capacity; Triploid; Growth; Oysters; Crassostrea gigas; Composition biochimique; Survie; Capacité trophque; Triploïdes; Croissance; Huîtres; Crassostrea gigas. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/1996/publication-3088.pdf |
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| Registros recuperados: 76 | |
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