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Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1 Ciência Rural
Cilento,Maria do Carmo; Pituco,Edviges Maristela; Jordão,Ricardo Spacagna; Ribeiro,Cláudia Pestana; Marchiori Filho,Moacir; Montassier,Hélio José.
An experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-1; Immunoglobulin isotypes; Experimental vaccine.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200021
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