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Public-domain software for root image analysis Rev. Bras. Ciênc. Solo
Costa,Mirian Cristina Gomes; Cunha,Isabela Maria de Lima; Jorge,Lúcio André de Castro; Araújo,Isabel Cristina da Silva.
In the search for high efficiency in root studies, computational systems have been developed to analyze digital images. ImageJ and Safira are public-domain systems that may be used for image analysis of washed roots. However, differences in root properties measured using ImageJ and Safira are supposed. This study compared values of root length and surface area obtained with public-domain systems with values obtained by a reference method. Root samples were collected in a banana plantation in an area of a shallower Typic Carbonatic Haplic Cambisol (CXk), and an area of a deeper Typic Haplic Ta Eutrophic Cambisol (CXve), at six depths in five replications. Root images were digitized and the systems ImageJ and Safira used to determine root length and surface...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Root length; Root surface area; Image processing; Root system.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832014000500001
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Grapevine root distribution in drip and microsprinkler irrigation Scientia Agricola
Bassoi,Luis Henrique; Hopmans,Jan W.; Jorge,Lúcio André de Castro; Alencar,Cristina Miranda de; Silva,José Antonio Moura e.
Grape (Vitis vinifera L.) yield and its quality are dependent of the status of the root system. Root distribution information is also valuable for soil and water management. An analysis of methods to evaluate the root distribution of grapevines for both, drip and microsprinkler irrigation in a Typic Acrustox is presented for the table grape cv. Italia grafted on the rootstock IAC-313, in northeastern Brazil. Measured root parameters using the monolith method were root dry weight (Dw) and root length density (Lv), while root area (Ap) was estimated using the soil profile method in combination with digital image analysis. For both irrigation systems, roots were present to the 1 m soil depth and extended laterally to 1 m distance from the trunk, but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis vinifera L.; Root system; Digital image.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200024
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Measuring bacterial cells size with AFM BJM
Osiro,Denise; Bernardes Filho,Rubens; Assis,Odilio Benedito Garrido; Jorge,Lúcio André de Castro; Colnago,Luiz Alberto.
Atomic Force Microscopy (AFM) can be used to obtain high-resolution topographical images of bacteria revealing surface details and cell integrity. During scanning however, the interactions between the AFM probe and the membrane results in distortion of the images. Such distortions or artifacts are the result of geometrical effects related to bacterial cell height, specimen curvature and the AFM probe geometry. The most common artifact in imaging is surface broadening, what can lead to errors in bacterial sizing. Several methods of correction have been proposed to compensate for these artifacts and in this study we describe a simple geometric model for the interaction between the tip (a pyramidal shaped AFM probe) and the bacterium (Escherichia coli JM-109...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atomic force microscopy (AFM); Escherichia coli; Cell dimensions; Bacteria visualization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100040
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