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Cadmium effect on the association of jackbean (Canavalia ensiformis) and arbuscular mycorrhizal fungi Scientia Agricola
Andrade,Sara Adrián López de; Jorge,Renato Atílio; Silveira,Adriana Parada Dias da.
The effect of cadmium (Cd) on mycorrhizal association and on shoot and root Cd concentration was investigated in jackbean plants under hydroponic conditions. The treatments consisted of the inoculation of three different species of arbuscular mycorrhizal fungi (AMF), Glomus etunicatum, G. intraradices and G. macrocarpum, and a non-inoculated control, two Cd (0 and 5 µmol L-1) and two P (1 and 10 mg L-1) levels in the nutrient solution. Mycorrhizal colonization, length of AMF extraradical mycelium, guaiacol peroxidase activity in roots, plant growth and root and shoot Cd and P concentrations were determined. Mycorrhizal status did not promote jackbean growth but in most of the cases mycorrhization increased root and shoot Cd concentrations. Cd ions were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Cd accumulation; Extraradical mycelium; Phytoremediation; Hydroponics.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400013
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Evaluation of the performance of different plastics used to seal nylon cDNA arrays Ciência e Agrotecnologia
Costa Netto,Antônio Paulino da; Drummond,Rodrigo Duarte; Felix,Juliana de Maria; Jorge,Renato Atílio; Menossi,Marcelo.
cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal detection; Nylon arrays; Sensitivity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700031
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