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| Kanitz,Fabio Adriano; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Batista,Helena Beatriz de Carvalho Ruthner; Flores,Eduardo Furtado. |
| ABSTRACT: This study investigated the suitability of virus isolation (VI) in mouse neuroblastoma cells (N2A) and baby hamster kidney cells (BHK-21) as a confirmatory test for diagnosis of bovine rabies. Fourty-eight brain samples from cattle suspected of rabies were initially submitted to fluorescent antibody test (FAT) and mouse inoculation test (MIT) for routine diagnostic. Subsequently, these specimens were submitted to three protocols of VI in each cell line: a single 24h or 72h passage (T1, T2), or three 48h passages (T3). The FAT and MIT combined detected 32/48 positive samples, from which MIT detected 32 and FAT 31. The average time required for final MIT results was 12.3 days (8 - 21). VI in BHK-21 cells provided definitive, positive results in... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Rabies; Cattle; Outbreaks; Diagnostic; Virus isolation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202193 |
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