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Cholesterol induces fetal rat enterocyte death in culture BJMBR
Gazzola,J.; Silva,É.P.P.; Kanunfre,C.C.; Verlengia,R.; Vecchia,M.G.; Curi,R..
The effect of cholesterol on fetal rat enterocytes and IEC-6 cells (line originated from normal rat small intestine) was examined. Both cells were cultured in the presence of 20 to 80 µM cholesterol for up to 72 h. Apoptosis was determined by flow cytometric analysis and fluorescence microscopy. The expression of HMG-CoA reductase and peroxisome proliferator-activated receptor gamma (PPARgamma) was measured by RT-PCR. The addition of 20 µM cholesterol reduced enterocyte proliferation as early as 6 h of culture. Reduction of enterocyte proliferation by 28 and 41% was observed after 24 h of culture in the presence and absence of 10% fetal calf serum, respectively, with the effect lasting up to 72 h. Treatment of IEC-6 cells with cholesterol for 24 h raised...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fetal enterocytes; Cholesterol; Intestine; IEC-6 cells; HMG-CoA; PPARgamma.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700019
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