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Bio-fortification and isotopic labelling of Se metabolites in onions and carrots following foliar application of Se and 77Se Organic Eprints
Kapolna, Emese; Laursen, Kristian Holst; Husted, Søren; Larsen, Erik H..
The aims were to bio-fortify onions by foliar application of selenium (Se) and to intrinsically label bioactive Se-metabolites in onion and carrot by enriched, stable77Se for use in human physiological studies. Onion bulbs and leaves were enriched in Se by repeated foliar spraying of 10 or 100 μg Se mL-1 solutions of sodium selenite (Se(IV)) or sodium selenate (Se(VI)). ICP-MS analysis of onion leaves and bulbs showed that the Se concentration was enhanced by up to a factor of approximately 50 and 200 in bulbs and leaves, respectively. HPLC-ICP-MS analysis of proteolytic plant extracts showed that foliar application of Se(IV) gave rise to bio-synthesis of a higher fraction of the desired organic Se species and was better tolerated by the plants than...
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health Crop health; Quality; Protection.
Ano: 2011 URL: http://orgprints.org/19812/1/FOODCHEM-D-11-03087R1.pdf
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Effect of foliar application of selenium on its uptake and speciation in carrot Organic Eprints
Kapolna, Emese; Hillestrøm, Peter; Laursen, Kristian; Husted, Søren; Larsen, Erik H..
Carrot (Daucus carota) shoots were enriched by selenium using foliar application. Solutions of sodium selenite or sodium selenate at 10 and 100 lg Se ml�1, were sprayed on the carrot leaves and the selenium content and uptake rate of selenium were estimated by ICP–MS analysis. Anion and cation exchange HPLC were tailored to and applied for the separation of selenium species in proteolytic extracts of the biological tissues using detection by ICP–MS or ESI–MS/MS. Foliar application of solutions of selenite or selenate at 100 lg Se ml�1 resulted in a selenium concentration of up to 2 lg Se g�1 (dry mass) in the carrot root whereas the selenium concentration in the controls was below the limit of detection at 0.045 lg Se g�1 (dry mass)....
Tipo: Journal paper Palavras-chave: Farming Systems; Food systems.
Ano: 2009 URL: http://orgprints.org/19083/4/19083.pdf
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SE-ENRICHMENT OF CARROT AND ONION VIA FOLIAR APPLICATION Organic Eprints
Kapolna, Emese; Laursen, Kristian H.; Hillestrøm, Peter; Husted, Søren; Larsen, Erik H..
The aim of this work was to study the selenium accumulation in carrot and onion plants using foliar application by sodium selenite and sodium selenate. Furthermore, we aimed at identifying the Se species biosynthesised by onion and carrot plants. The results were used to prepare for production of 77Se enriched plants for an ongoing human absorption study.
Tipo: Conference paper, poster, etc. Palavras-chave: Farming Systems; Food systems.
Ano: 2008 URL: http://orgprints.org/19084/2/19084.pdf
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The OrgTrace project: Content, Bioavailability and Health Effects of Trace Elements and Bioactive Components of Food Products Cultivated in Organic and Conventional Agricultural Systems Organic Eprints
Husted, Søren; Laursen, Kristian Holst; Larsen, Erik Huusfeldt; Kapolna, Emese; Knuthsen, Pia; Søltoft, Malene; Bügel, Susanne; Mark, Alicja Budek; Lauridsen, Charlotte; Jacobsen, Maja; Jørgensen, Henry; Halekoh, Ulrich; Kristensen, Kristian.
Trace elements, bioactive secondary metabolites and vitamins are among the most important quality parameters in plants. Yet, very little information is available on their content, bioavailability and health effects in organically grown plant food products. The main objective of OrgTrace is to study the impact of different agricultural management practices relevant for organic farming on the ability of cereal and vegetable crops to absorb trace elements from the soil and to synthesize bioactive compounds (secondary metabolites, antioxidant vitamins and phytates) with health promoting effects. Based on different plant products produced in OrgTrace, diets were composed and the bioavailabilities of health promoting substances were analyzed in a human...
Tipo: Conference paper, poster, etc. Palavras-chave: "Organics" in general; Farming Systems; Soil; Food systems.
Ano: 2011 URL: http://orgprints.org/18877/4/18877.pdf
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