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	Provedor de dados BJM (2) Autor Kawaguti,Haroldo Y. (2) Sato,Hélia H. (2) Fleuri,Luciana F. (1) Orsi,Daniela C. (1) Palavra-chave Cell immobilization (1) Cellulosimicrobium cellulans (1) Chitinase (1) Fungal lysis (1) Glucosyltransferase (1) Klebsiella sp (1) Palatinose (1) Protoplasts (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using immobilized cells BJM Orsi,Daniela C.; Kawaguti,Haroldo Y.; Sato,Hélia H.. The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell immobilization; Glucosyltransferase; Klebsiella sp; Palatinose; Response surface methodology. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100010 Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 BJM Fleuri,Luciana F.; Kawaguti,Haroldo Y.; Sato,Hélia H.. This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Cellulosimicrobium cellulans; Fungal lysis; Protoplasts. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 Registros recuperados: 2 Primeira ... 1 ... Última

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