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| Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T. |
| Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
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