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Anticancer properties of phospholipase A2 fromDaboia siamensis venom on human skin melanoma cells J. Venom. Anim. Toxins incl. Trop. Dis.
Khunsap,Suchitra; Khow,Orawan; Buranapraditkun,Supranee; Suntrarachun,Sunutcha; Puthong,Songchan; Boonchang,and Supatsorn.
Abstract Background Phospholipase A2 (PLA2) is a major component of theDaboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28). Methods dssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined. Results dssPLA2 exhibited cytotoxicity on SK-MEL-28 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phospholipase A2; Daboia siamensis venom; Skin melanoma; Anticancer.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100305
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