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| Sathiyaraj,Gayathri; Srinivasan,Sathiyaraj; Kim,Ho-Bin; Subramaniyam,Sathiyamoorthy; Lee,Ok Ran; Kim,Yeon-Ju; Yang,Deok Chun. |
| Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. A Taguchi's orthogonal array experimental design was applied to optimize the preliminary production of polygalacturonase (PG) and pectin lyase (PL) using submerged culture condition. This method was applied to evaluate the significant parameters for the production of enzymes. The process variables were pH, pectin concentration, incubation time and temperature. Optimization of process parameters resulted in high levels of enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of 1.5% pectin. Among different nitrogen sources, urea and peptone showed high production of PG and PL, respectively. The enzyme production and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. destructans; Polygalacturonase; Pectin lyases; Ginseng. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200048 |
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