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| Goto, Kohta; Fukuda, Kenji; Senda, Akitsugu; Saito, Tadao; Kimura, Kazumasa; Glander, Kenneth E.; Hinde, Katie; Dittus, Wolfgang; Milligan, Lauren A.; Power, Michael L.; Oftedal, Olav T.; Urashima, Tadasu; 浦島, 匡; 福田, 健二. |
| Human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. Neutral and acidic oligosaccharides were isolated from the milk of three species of Old World or catarrhine monkeys (Cercopithecidae: rhesus macaque (Macaca mulatta), toque macaque (Macaca sinica) and Hamadryas baboon (Papio hamadryas)) and three of New World or platyrrhine monkeys (Cebidae: tufted capuchin (Cebus apella)and Bolivian squirrel monkey (Saimiri boliviensis); Atelidae: mantled howler (Alouatta palliata)). The milks of these species contained 6-8% total sugar, most of which was lactose: the estimated ratio of oligosaccharides to lactose in Old World monkeys (1:4 to... |
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Palavras-chave: Old world monkey; New world monkey; Milk oligosaccharide; Rhesus macaque; Toque macaque; Baboon; Capuchin; Mantled howler; Squirrel monkey; N-glycolylneuraminic acid. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3144 |
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| Asakuma, Sasaki; Yokoyama, Tomoko; Kimura, Kazumasa; Watanabe, Yoko; Nakamura, Tadashi; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| It is believed that human milk oligosaccharides (HMO) act as receptor analogues that inhibit the attachment of pathogenic microorganisms to the infant colon, but the direct involvement of HMO in anti infection has not as yet been demonstrated. The present study was conducted to clarify whether HMOs as well as commercially available galacto oligosaccharides (GO), from which lactose has been removed, affect the expression of toll-like receptor (TLR) 2 and 4, and of MD2 mRNA in the HT-29, human colonic cell line. HT-29 cells were treated with neutral human milk oligosaccharides (nHMOs), which had been separated from the acidic oligosaccharide fraction and also from lactose, as well as with GOs. HT-29 cells were treated with specific oligosaccharides of major... |
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Palavras-chave: Human milk oligosaccharides; Galacto-oligosaccharides; Toll-like receptor; Intestinal cell; HT-29 cells. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4184 |
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| Sumiyoshi, Wataru; Urashima, Tadasu; Nakamura, Tadashi; Arai, Ikichi; Nagasawa, Takashi; Saito, Tadao; Tsumura, Norihiko; Wang, Bing; Brand-Miller, Janette; Watanabe, Yoko; Kimura, Kazumasa; 住吉, 渉; 浦島, 匡; 中村, 正; 荒井, 威吉; 長澤, 孝志; 齋藤, 忠夫; 津村, 宣彦; 渡邉, 陽子; 木村, 一雅. |
| The concentrations of the six major sialyl oligosaccharides found in the milk of 24 Japanese women were determined using reverse phase high performance liquid chromatography of the 3-methyl-1-phenyl-5- pyrazolone derivatives of the oligosaccharides. The milk was collected at 4, 10, 30 and 100 days postpartum. The concentrations of all six oligosaccharides decreased during the course of lactation. The concentrations of 6'-sialyllactose, sialyllacto-N-tetraose b and disialyllacto-N-tetraose decreased only after 10 days postpartum, whereas those of sialyllacto-N-tetraose a and sialyllacto-N-neotetraose c as well as 3'-sialyllactose decreased from 4 days postpartum. Large differences were observed between present data and those previously reported for Italian... |
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Palavras-chave: Human milk; Sialyl oligosaccharide; Lactation period; PMP; Reverse phase HPLC. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3287 |
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