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Pratlong, M.; Haguenauer, A.; Chabrol, O.; Klopp, C.; Pontarotti, P.; Aurelle, D.. |
The question of species survival and evolution in heterogeneous environments has long been a subject for study. Indeed, it is often difficult to identify the molecular basis of adaptation to contrasted environments, and nongenetic effects increase the difficulty to disentangle fixed effects, such as genetic adaptation, from variable effects, such as individual phenotypic plasticity, in adaptation. Nevertheless, this question is also of great importance for understanding the evolution of species in a context of climate change. The red coral (Corallium rubrum) lives in the Mediterranean Sea, where at depths ranging from 5 to 600m, it meets very contrasted thermal conditions. The shallowest populations of this species suffered from mortality events linked... |
Tipo: Text |
Palavras-chave: Cnidarians; Gene expression; Local adaptation; Octocoral polymorphism; Transcriptome. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00371/48257/48661.pdf |
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Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.. |
Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf |
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