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Krawutschke, Manuel; Weiher, Nina; Gierus, Martin; Thaysen, Johannes; Taube, Friedhelm. |
Ensilage of red clover results in lower concentrations of non-protein nitrogen compared to other forage legumes. It is assumed that the polyphenol oxidase (PPO) activity in red clover is related to this effect. Therefore, a laboratory ensiling trial was carried out to quantify the changes in crude protein (CP) fractions during the ensiling process using the Cornell Net Carbohydrate and Protein System. At four cutting dates, fresh, wilted and ensiled herbage of three red clover genotypes and white clover grown in two systems (with and without mechanical stress) was sampled. The specific PPO activity was measured photometrically in fresh leaves of the plants. Concentrations of fraction A increased from 130-197 g/kg CP to 372-544 g/kg CP during the... |
Tipo: Conference paper, poster, etc. |
Palavras-chave: Pasture and forage crops. |
Ano: 2011 |
URL: http://orgprints.org/17345/3/Krawutschke_17345.pdf |
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Weiher, Nina; Krawutschke, Manuel; Gierus, Martin; Taube, Friedhelm. |
Increasing protein quality of forages in ruminant nutrition may contribute to reduce nitrogen losses in specialized dairy farms in Europe. Red clover is known to have high polyphenol oxidase (PPO) activity among forage legumes. The enzyme oxidizes phenols to quinones. These highly reactive quinones form stabilized quinone-protein-complexes, protecting protein from fast degradation. The objective of this study was to investigate the effect of the specific PPO activity on the protein fractions of twelve red clover genotypes in two management systems (without and with mechanical stress) in pure red clover swards. The results showed that genotype seem to be of minor importance compared to the variation in PPO activity induced by mechanical stress and... |
Tipo: Conference paper, poster, etc. |
Palavras-chave: Pasture and forage crops. |
Ano: 2011 |
URL: http://orgprints.org/17397/3/Weiher_17397.pdf |
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