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Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP Genet. Mol. Biol.
Raut,Ashwin A.; Kumar,Anil; Kala,Sheo N.; Chhokar,Vinod; Rana,Neeraj; Beniwal,Vikas; Jaglan,Sundeep; Samuchiwal,Sachin K.; Singh,Jitender K.; Mishra,Anamika.
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; DGAT1; PCR-SSCP; Polymorphism; SNP.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400011
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Acoustic communication in the Red-vented Bulbul Pycnonotus cafer Anais da ABC (AABC)
Kumar,Anil.
This paper deals with acoustic communication in the Red-vented Bulbul Pycnonotus cafer. This species emits a high variety of vocal signals that can be classified on the basis of their acoustical features and context of production. Individuals sang throughout the year and most songs were discrete and stereotyped. The songs were composed of strophes (phrases) with minor structural variations of elements that were preceded and followed by a temporal gap (3 to 12s). Most strophes were composed of 2 to 6 elements that were often dissimilar in structure and ranging from 0.98 to 4.5 kHz. The biological function of the song appeared to be to maintain pair bonds and to synchronize breeding activities. Different types of context-specific calls were identified....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acoustic communication; Pycnonotus cafer; Bird song; Alarm calls; Roosting calls; Flight calls.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000200024
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Validation of computationally predicted substrates for laccase BABT
Reena,; Dhall,Purnima; Kumar,Rita; Kumar,Anil.
Present study reports the validation (oxidation) of computationally predicted oxidation of xenobiotic contaminants by commercially available pure laccase from Trametes versicolor. Selected contaminants were predicted as potential targets for laccase oxidation by using in-silico docking tool. The oxidation by laccase was measured by change in absorbance at specific λ max of each compound. Sinapic acid and tyrosine were taken as positive and negative controls, respectively. Oxidation was observed in m-chlorophenol, 2,4 di-chlorophenol, 2,4,6 tri-chlorophenol, captan, atrazine and thiodicarb, except malathion, which showed no activity. It could be speculated that the predicted substrates showing oxidation shared homology at structural and chemical level with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; In silico screening; Laccase; Xenobiotics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500803
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