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| HE,Dongliang; ZHANG,Zhijun; LI,Huizhen; XIA,Yaoyao; LI,Xiaojun; CHEN,Tie. |
| Abstract Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters of perilla protein isolate (PPI), which has functional properties. A central composite experimental design was applied to investigate the effects of three factors (ultrasonic power, extraction time, and liquid-to-solid ratio) on six responses: protein yield, water solubility index (WSI), water absorption capacity (WAC), oil holding capacity (OHC), emulsifying activity (EA), and foaming ability (FA). Based on the RSM results, the optimum conditions were an ultrasonic power of 206 W, and extraction time of 32.4 min, and a liquid-to-solid ratio of 10.34:1. Under these conditions, PPI had high protein yield (26.1%) and functional properties (55.2%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrasound-assisted extraction; Perilla protein isolate; Protein yield; Functional properties; Thermal stability; Polypeptide profile. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000200348 |
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| LI,Huizhen; ZHANG,Hongjiao; ZHANG,Zhijun; CUI,Lixia. |
| Abstract A Box-Behnken design was employed to optimize ultrasound-assisted enzymatic extraction of polysaccharides from perilla leaves. Four independent variables, namely, liquid-to-solid ratio, enzymatic time, enzymatic temperature, and ultrasonic power were investigated. Under the optimal liquid-to-solid ratio of 41:1, enzymatic time of 40 min, enzymatic temperature of 49 °C, and ultrasonic power of 204 W, the experimental polysaccharide yield was 3.84% (n = 3), which was close to the value predicted by response surface methodology (3.9%). Four polysaccharides (PLP1, PLP2, PLP3, and PLP4) were obtained with DEAE-cellulose-52 and Sephadex G-100 chromatography, and their antioxidant activities were investigated with various antioxidant assays in vitro. All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Perilla leaves; Polysaccharide; Ultrasound-assisted enzymatic extraction; Antioxidant activities. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100036 |
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| LI,Huizhen; ZHANG,Zhijun; HE,Dongliang; XIA,Yaoyao; LIU,Qingye; LI,Xiaojun. |
| Abstract Response surface methodology (RSM) was used to optimize ultrasound-assisted aqueous enzymatic extraction (UAAEE) conditions for perilla seed oil. Under the optimum conditions—which were a liquid-to-solid ratio of 4.4:1, a hydrolysis time of 2.66 h, a hydrolysis temperature of 50.87 °C and an ultrasound treatment time of 24.74 min—an oil yield of 31.34% was obtained. Comparisons of the physicochemical characteristics of oil obtained using UAAEE with those of oil obtained by solvent extraction (SE oil) and cold pressing extraction (CPE oil) revealed similar refractive indices and saponification values, but UAAEE oil had a higher iodine value and better stability against oxidation, with a low peroxide value. UAAEE oil contained higher levels of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Perilla seed oil; Ultrasound-assisted aqueous enzymatic extraction; Physical and chemical characteristics; Fatty acid composition; Antioxidant activities. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500071 |
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