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Registros recuperados: 103
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Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum ArchiMer
Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher.
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment...
Tipo: Text Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf
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Kystes modernes de Dinoflagellés en Baie de Vilaine-Bretagne Sud (France) ArchiMer
Larrazabal, Me; Lassus, Patrick; Maggi, Pierre; Bardouil, Michele.
A preliminary study of modern marine dinoflagellate cysts has been performed in Vilaine Bay through winters 1986, 1987 and 1989. Direct observations of muddy sand samples and most probable number (MPN) method applied to cysts have evidenced a low win ter species diversity with Spiniferites spp. and Scrippsiella sp. cysts as predominant species. Change in relative importance of maximum species diversity area from ycar to year suggests a possible action of bottom currents. A study of dormant stages of toxie dinoflagellates, genus Alexandrium and Dinophysis, has corroborated the occurrence of A. minutum cysts (IO to 30 cysts.g-I sediment) at low amounts and absence of Dinophysis sp. resting stages. More generally, comparison of cysts in sediments with summer...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00133/24472/22488.pdf
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Mise à jour des données sur les organismes responsables d'eaux colorées - Extension au microplancton produisant des toxines ArchiMer
Lassus, Patrick.
Cette mise à jour (*) constitue avant tout un document de travail, à charge pour les utilisateurs de compléter les données présentées ici par de nouvelles fiches de leur propre factures, s'ils le désirent. La présentation sous forme de classeur à justement pour but de faciliter cette mise à jour. Afin d'éviter d'inutiles prolégomènes nous renvoyons au "catalogue descriptif des principaux organismes responsables d'eaux rouges" pour tout renseignement sur les méthodes de prélèvements et d'examen du phytoplancton ainsi que sur les bases de la systématique des péridiniens.
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Ano: 1980 URL: http://archimer.ifremer.fr/doc/00046/15733/13135.pdf
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Application of a digital pattern recognition system to Dinophysis acuminata and D-sacculus complexes ArchiMer
Truquet, Philippe; Lassus, Patrick; Honsell, Giorgio; Le Dean, Loic.
An image discrimination technique was developed to improve specific identification of some toxic Dinophysis cells (marine dinoflagellates involved in diarrhoeic shellfish poisoning), especially the ''acuminata'' and ''sacculus'' groups, which can be present at different ratios in natural sea-water samples collected during toxic episodes. This work was performed with image analysis software SAMBA (TITN Alcatel) using preserved cells directly observed through an inverted microscope and recorded with a video camera before further processing. All morphometric parameters used for discrimination of 7 different species or morphotypes of Dinophysis were tested with discriminant analysis. This study indicates that Dinophysis sp. and D. pavillardi seem well...
Tipo: Text Palavras-chave: Dinophysis sp.; Analyse d'image; Taxonomie; Phytoplancton; Dinophysis sp; Image analysis; Taxonomy; Phytoplankton.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00188/29890/28337.pdf
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Spirolide uptake and detoxification by Crassostrea gigas exposed to the toxic dinoflagellate Alexandrium ostenfeldii ArchiMer
Medhioub, Walid; Lassus, Patrick; Truquet, Philippe; Bardouil, Michele; Amzil, Zouher; Sechet, Veronique; Sibat, Manoella; Soudant, Philippe.
Oysters (Crassostrea gigas) were exposed 4 days to cultures of the toxic dinoflagellate Alexandrium ostenfeldii (strain CCMP1773) that produces spirolides belonging to fast acting toxins (FAT) and let depurate for 7 days. During depuration, oysters were either fed the non-toxic algae Isochrysis galbana Tahitian clone (T. Iso) or starved. The objectives of this experiment were to evaluate (i) spirolide uptake and depuration by oysters (ii) spirolide effects on oysters and (iii) oyster recovery according to food supply during depuration. A. ostenfeldii cells were filtered and ingested by oysters while faeces contained numerous intact cells of the toxic diet. This suggested that ingested cells were not totally digested by oysters. Contents of spirolides (SPX)...
Tipo: Text Palavras-chave: Spirolides; Alexandrium ostenfeldii; Contamination; Depuration; Crassostrea gigas; Inflammatory responses.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20953/18740.pdf
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Influence of environmental parameters on Karenia selliformis toxin content in culture ArchiMer
Medhioub, Amel; Medhioub, Walid; Amzil, Zouher; Sibat, Manoella; Bardouil, Michele; Ben Neila, Idriss; Mezghani, Salah; Hamza, Asma; Lassus, Patrick.
Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabes gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae oil enriched Gaillard f/2 medium. The influence of growing conditions oil toxin content was studied, examining the effects of (i) different culture Volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15 degrees C et 20-21 degrees C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 +/- 0.14 div d(-1)) was obtained at 20 degrees C...
Tipo: Text Palavras-chave: Mouse bioassay; Gymnodimine; Toxicity; Growth; Karenia selliformis.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7383.pdf
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Déterminisme des floraisons de dinophysis en zone côtière ArchiMer
Lassus, Patrick; Proniewski, Frédéric.
Depuis 1983, des intoxications diarrhéiques observées chez des consommateurs de moules, en Bretagne sud et en Normandie, ont pu être corrélées avec la présence estivale dans les eaux côtières d'un dinoflagellé appartenant au genre Dinophysis. Des résultats similaires avaient auparavant été signalés sur les côtes hollandaises, japonaises, espagnoles par et norvégiennes. Les espèces incriminées sont diverses: Dinophysis fortii, D. acuminata, D. acuta et D. norvegica, avec une composition toxinique variable selon les proportions d'acide okadaïque et de ses dérivés méthylés. Récemment l'espèce D. acuminata considérée comme responsable de ces intoxications (DSP : Diarrheic Shellfish Poison) sur les côtes françaises a été réexaminée sur le plan de sa diagnose et...
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Ano: 1988 URL: http://archimer.ifremer.fr/doc/00142/25336/23413.pdf
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Influence de la température et de la salinité sur les perturbations phytoplanctoniques observées dans le Mor Bras en 1983 et 1984 ArchiMer
Truquet, Isabelle; Lassus, Patrick; Truquet, Philippe; Maggi, Pierre.
L'équilibre des écosystèmes phytoplanctoniques sur le littoral sud breton a été modifié à deux reprises, en 1983 et 1984, et selon une chronologie apparemment identique, à savoir: des eaux colorées (plus de 106 cellules par litre) à diatomées apparaissant soit en mars-avril (Skeletonema costatum) soit en juin (Nitzschia, Rhizoselenia et Chaetoceros) et suivies dans le second cas par des concentrations inhabituelles de certaines espèces de dinoflagellés.
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Ano: 1985 URL: http://archimer.ifremer.fr/doc/00139/25016/23120.pdf
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Influence de la Chloration sur les Premiers Stades Larvaires de Palaemon serratus (Pennant). ArchiMer
Lassus, Patrick; Maggi, Pierre; Le Baut, Claire; Bardouil, Michèle; Bocquene, Gilles; Le Dean, Loïc; Truquet, Philippe.
In order to estimate the effects of chlorine used in cooling systems of electric power plants on zooplankton, some experiments have been realized with the four first larval stages of the prawn (P. serratus Pennant). Chlorine injection was performed by constant adding at 16 and 20 degree C with times exposures of 5, 15 and 25 minutes. If there is no detectable variations in sensitivity of different stages, toxic effects of oxydants issued from chlorination can be observed (for both the two acclimatation temperatures) at 15 minutes exposure with a LD 50 : 32 mg/l of chlorine.
Tipo: Text Palavras-chave: Palaemon serratus; Pollution effects; Larval development; Chlorine; Toxicity.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-1886.pdf
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Dinophysis acuminate (dinophyceae), une espèce à géométrie variable sur les côtes françaises ArchiMer
Lassus, Patrick; Marcaillou-le Baut, Claire.
Tipo: Text Palavras-chave: Morphologie; Variabilité; Ultrastructure; Croissance; France; Dinophysis acuminata; Europe; Milieu marin; Zone côtière; Phytoplancton; Pyrrophycophyta; Algae; Thallophyta; Morphology; Variability; Ultrastructure; Growth; France; Europe; Marine environment; Coastal zone; Phytoplankton; Pyrrophycophyta; Algae; Thallophyta.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00133/24468/22484.pdf
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Nuisances et intoxications causées en France par le phytoplancton et les "efflorescences" marines - Synthèse préliminaire ArchiMer
Sournia, A.; Belin, Catherine; Berland, B.; Erard-le Denn, Evelyne; Grzebyk, Daniel; Marcaillou-le Baut, Claire; Lassus, Patrick; Partensky, F..
Les intoxications humaines résultant de la consommation de coquillages sont connues en France depuis plusieurs siècles. On peut supposer que la cause principale a d'abord été d'origine bactériologique, eu égard aux conditions de transport et à l'absence de contrôles de salubrité dans le passé. Quoi qu'il en soit, il faut compter aujourd'hui avec une autre origine, à savoir les proliférations de certaines algues unicellulaires planctoniques (phytoplancton), qui causent dans notre pays jusqu'à plusieurs milliers d'intoxications par an. Soulignons que la France a été jusqu'ici largement épargnée, puisque d'autres pays comptabilisent des dizaines ou centaines de décès depuis le début du siècle. On vient toutefois de découvrir la présence dans nos eaux côtières...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00069/18017/15569.pdf
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Phytoplancton des zones myticoles de la baie de Vilaine et intoxication par les coquillages. ArchiMer
Andresen Leitao, Maria Da Paz; Lassus, Patrick; Maggi, Pierre; Le Baut, Claire; Chauvin, Jacky; Truouet, Philippe.
Following 1978 and 1981 human poisoning by mussels in Vilaine Bay (France) a survey of potentially toxic dinoflagellates was conducted in that area in 1982. At the same time paralytic shellfish poison (PSP) analyses were made, the authors studied the dinoflagellates encountered in the intestinal guts of mussels from January to August 1982. Results failed to demonstrate an occurrence of PSP producing species, either by toxicological analysis or dinoflagellate persistency in the mussel guts.
Tipo: Text Palavras-chave: Mytilus edulis; Bivalvia; Marine mollusks; Stomach content; Toxicity; Phytoplankton; Biological poisons; Mussel culture.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-1853.pdf
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Toxicité des moules : étude de la décontamination PSP et DSP in vitro et in situ ArchiMer
Lassus, Patrick; Berthome, Jean-paul.
Laboratory bioassays have been performed. using toxic P. tamarensis mass cultures (MOG 835), in order to assess potential depuration kinetics of PSP contamined cultured blue mussels. Besides, from fields observations and translocation experiences to safe area, we have seeked for probable depuration kinetics of shellfishes having accumulated during summer 1986 high levels of DSP producing Dinophysis. Such preliminary studies may drive to further experiences giving more accuracy to the establishment of thresholds recquired by shellfishes depuration policy.
Tipo: Text Palavras-chave: Moules; PSP; DSP; Protogonyaulax tamarensis; Dinophysis; Décontamination; Blue mussels; PSP; DSP; Protogonyaulax tamarensis; Dinophysis; Depuration.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00119/23020/20851.pdf
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Catalogue descriptif des principaux organismes responsables d'eaux rouges ArchiMer
Lassus, Patrick.
Les phénomènes de coloration des eaux marines au voisinage des côtes, des estuaires, à l'intérieur des baies, lagunes ou ouvrages portuaires, sont connus depuis longtemps. Neanmoins, leur fréquence augmentant depuis quelques années sur le littoral français, des risques d'intoxications par ingestion de bivalves contaminés similaires à celles que connaissent régulièrement les Etats-Unis et le Canada, sont à craindre. De fait, si bon nombre de ces phénomènes "d'eaux rouges" restent sans effet après leur disparition, certains, par contre, peuvent entraîner des mortalités d'animaux marins, ou pire encore, de graves empoisonnements chez les consommateurs de "fruits de mer", du fait de l'énorme pouvoir concentrateur des bivalves.
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Ano: 1980 URL: http://archimer.ifremer.fr/doc/00046/15774/13176.pdf
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Paralytic shellfish poison accumulation yields and feeding time activity in the Pacific oyster (Crassostrea gigas) and king scallop (Pecten maximus) ArchiMer
Bougrier, Serge; Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Truquet, Philippe.
Pacific oysters and king scallops placed individually in a recirculating flume were fed for 2 weeks with a constant concentration (120 cell ml(-1)) of a toxic strain of Alexandrium minutum. Fluorescence at the outlet of each experimental unit was measured continuously, and biodeposits were recovered twice daily to evaluate feeding time activity (FTA) and rates of organic filtration (OFR), ingestion (OIR) and organic absorption (OAR). Ion-pairing high performance liquid chromatography (IP-HPLC) was performed concurrently to quantify paralytic phycotoxins both (i) individually in shellfish at the end of the contamination period and (ii) in A. minutum cultures to estimate cellular toxin concentration. These data allowed comparison of the actual tissue toxin...
Tipo: Text Palavras-chave: Alexandrium minutum; Crassostrea gigas; Feeding activity; Paralytic shellfish poisoning.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-576.pdf
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Improving detoxification Efficiency of PSP-contaminated oysters (Crassostrea gigas Thunberg) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp.
Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large...
Tipo: Text Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf
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Evaluation du potentiel nutritif des eaux de la baie de Vilaine pendant l'été 1987 ArchiMer
Le Dean, Loic; Truquet, Philippe; Lassus, Patrick.
The growth potential study of waters of the Vilaine bay was realized from march to september 1987 and for three sampling stations. The growth of depleted test cells of Skeletonema costatum was estimated and compared from existing nutrients pools for aIl water samples. As a first result it appears that inorganic nitrogen could stimulate the growth during spring while no clear relationship is observed between the algal growth and other hydrological parameters during spring or summer periods. Nevertheless these first results need further corroboration.
Tipo: Text Palavras-chave: Potentiel nutritif; Baie de Vilaine; Skeletonema costatum; Test biologique; Growth potential; Vilaine Bay; S. costatum; Bioassay.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25037/23146.pdf
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Gut passage times in two bivalve molluscs fed toxic microalgae: Alexandrium minutum, A-catenella and Pseudo-nitzschia calliantha ArchiMer
Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent.
The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (...
Tipo: Text Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf
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Recherche d'acide okadaïque (et de ses dérivés) chez les bactéries associées aux dinoflagellés responsables d'intoxication diarrhéique, sur les côtes françaises ArchiMer
Sechet, Veronique; Quilliam, Michael; Lassus, Patrick.
L'hypothèse de la toxinogénèse bactérienne autonome dans le cadre des efflorescences marines toxiques de type DSP a été examinée. 18 souches bactériennes isolées à partir de culture de Prorocentrum lima et 12 souches obtenues à partir de prélèvements dans le milieu marin de Dinophysis sacculus (Baie de Vilaine) et de D. acuminata (Concarneau) ont été cultivées en milieu liquide, milieu de Shiba, pauvre en phosphore inorganique. La nouvelle espèce de bactérie présumée toxique, Roseobacter algicola, isolée préalablement à partir de P. lima, au laboratoire "Phycotoxines et Nuisances", a également été testée. Après extraction méthanolique et prépurification sur cartouches de silice, les résidus ont été estérifiés par le 9-antryldiazométhane afin d'être dosés...
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Ano: 1997 URL: http://archimer.ifremer.fr/doc/00104/21575/19155.pdf
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Why is Asari (= Manila) clam Ruditapes philippinarum fitness poor in Arcachon Bay: a meta-analysis to answer? ArchiMer
De Montaudouin, X.; Lucia, M.; Binias, C.; Lassudrie, Malwenn; Baudrimont, M.; Legeay, A.; Raymond, N.; Jude-lemeilleur, F.; Lambert, C.; Le Goic, N.; Garabetian, F.; Gonzalez, P.; Hegaret, Helene; Lassus, Patrick; Mehdioub, W.; Bourasseau, L.; Daffe, G.; Paul-pont, I.; Plus, Martin; Do, V. T.; Meisterhans, G.; Mesmer-dudons, N.; Caill-milly, Nathalie; Sanchez, Florence; Soudant, P..
Asari (= Manila) clam, Ruditapes philippinarum, is the second bivalve mollusc in terms of production in the world and, in many coastal areas, can beget important socio-economic issues. In Europe, this species was introduced after 1973. In Arcachon Bay, after a decade of aquaculture attempt, Asari clam rapidly constituted neo-naturalized population which is now fished. However, recent studies emphasized the decline of population and individual performances. In the framework of a national project (REPAMEP), some elements of fitness, stressors and responses in Arcachon bay were measured and compared to international data (41 publications, 9 countries). The condition index (CI=flesh weight/shell weight) was the lowest among all compared sites. Variation in...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Condition index; Perkinsus; Brown Muscle Disease; Element contamination; Immune system; Arcachon Bay.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00280/39123/37676.pdf
Registros recuperados: 103
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