Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Nature Precedings (2) Autor Laura Beatty (2) Jehonathan Pinthus (1) Jehonathan Pinthus (1) Jian-Ping Lu (1) JianPing Lu (1) Palavra-chave Biotechnology (2) Molecular Cell Biology (1) Tipo do documento Manuscript (2) Ano 2008 (2) País United Kingdom (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells Nature Precedings Jian-Ping Lu; Laura Beatty; Jehonathan Pinthus. Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform’s series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living... Tipo: Manuscript Palavras-chave: Biotechnology; Molecular Cell Biology. Ano: 2008 URL: http://precedings.nature.com/documents/1550/version/2 Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells Nature Precedings JianPing Lu; Laura Beatty; Jehonathan Pinthus. Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform's series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living... Tipo: Manuscript Palavras-chave: Biotechnology. Ano: 2008 URL: http://precedings.nature.com/documents/1550/version/1 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco