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	Provedor de dados 52 (3) Autor Lauretti,Flávio (3) Linhares,Rosa Elisa Carvalho (2) Nozawa,Carlos Mitihiko (2) Benati,Fabrício José (1) Bolognini,Andrea Maria (1) Botelho,Maria Valéria J. (1) Hasenack,Beatriz S. (1) Hashimoto,Newton (1) Linhares,Rosa Elisa C. (1) Melo,Fernando L. de (1) Meneghetti,Ângelo Cesar (1) Nozawa,Carlos M. (1) Orlandi,Janaina M. (1) Santos,Norma (1) Ueda,Marli (1) Mais... Palavra-chave Rotavirus (2) Cell culture (1) Concanavalin A (1) Diarrhea (1) Enzyme immunoassay (1) LA (1) PAGE (1) PCR (1) Poliovirus antigen (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2007 (1) 2002 (1) 2001 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Development of an enzyme immunoassay for poliovirus antigens 52 Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko. An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immunoassay; Poliovirus antigen. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005 Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997 52 Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko. Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006 The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture 52 Hasenack,Beatriz S.; Botelho,Maria Valéria J.; Lauretti,Flávio; Melo,Fernando L. de; Orlandi,Janaina M.; Linhares,Rosa Elisa C.; Ueda,Marli; Nozawa,Carlos M.. Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields. Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Concanavalin A; Cell culture. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003 Registros recuperados: 3 Primeira ... 1 ... Última

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