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	Provedor de dados 74 (2) Autor Cao,Bihao (2) Chen,Guoju (2) Lei,Jianjun (2) Chen,Changming (1) Chen,Qinghua (1) Hao,Xuefeng (1) Huang,Zhiyin (1) Mais... Palavra-chave Anther-specific (1) Barnase gene (1) CaMF3 (1) Capsicum annuum L. genic male sterility (1) Cre gene (1) Cre/loxP system (1) Eggplant (1) IAH1 (1) Male sterility (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Restoring pollen fertility in transgenic male-sterile eggplant by Cre/loxp-mediated site-specific recombination system 74 Cao,Bihao; Huang,Zhiyin; Chen,Guoju; Lei,Jianjun. This study was designed to control plant fertility by cell lethal gene Barnase expressing at specific developmental stage and in specific tissue of male organ under the control of Cre/loxP system, for heterosis breeding, producing hybrid seed of eggplant. The Barnase-coding region was flanked by loxP recognition sites for Cre-recombinase. The eggplant inbred/pure line ('E-38') was transformed with Cre gene and the inbred/pure line ('E-8') was transformed with the Barnase gene situated between loxp. The experiments were done separately, by means of Agrobacterium co-culture. Four T0-plants with the Barnase gene were obtained, all proved to be male-sterile and incapable of producing viable pollen. Flowers stamens were shorter, but the vegetative phenotype was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eggplant; Male sterility; Barnase gene; Cre gene; Cre/loxP system. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200017 Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L. 74 Hao,Xuefeng; Chen,Changming; Chen,Guoju; Cao,Bihao; Chen,Qinghua; Lei,Jianjun. Previous work on gene expression analysis based on RNA sequencing identified a variety of differentially expressed cDNA fragments in the genic male sterile-fertile line 114AB of Capsicum annuum L. In this work, we examined the accumulation of one of the transcript-derived fragments (TDFs), CaMF3 (male fertile 3), in the flower buds of a fertile line. The full genomic DNA sequence of CaMF3 was 1,951 bp long and contained 6 exons and 5 introns, with the complete sequence encoding a putative 25.89 kDa protein of 234 amino acids. The predicted protein of CaMF3 shared sequence similarity with members of the isoamyl acetate-hydrolyzing esterase (IAH1) protein family. CaMF3 expression was detected only in flower buds at stages 7 and 8 and in open flowers of a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther-specific; CaMF3; Capsicum annuum L. genic male sterility; IAH1. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500016 Registros recuperados: 2 Primeira ... 1 ... Última

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