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| Silva,Paulo da; Santos,Adolfo Carlos Barreto; Sato,Daisy Nakamura; Silva,Jaqueline Otero; Medeiros,Marta Inês Cazentini; Carneiro,Ana Maria Machado; Leite,Sergio Roberto de Andrade; Leite,Clarice Queico Fujimura. |
| INTRODUCTION: Rhodococcus equi is an opportunistic pathogen, causing rhodococcosis, a condition that can be confused with tuberculosis. Often, without identifying M. tuberculosis, physicians initiate empiric treatment for tuberculosis. R. equi and M. tuberculosis have different susceptibility to drugs. Identification of R. equi is based on a variety of phenotypic, chromatographic, and genotypic characteristics. OBJECTIVE: This study aimed to characterize bacterial isolates from sputum samples suggestive of R. equi. METHODS: The phenotypic identification included biochemical assays; thin-layer chromatography (TLC) and polymerase chain reaction (PCR) were used for genotypic identification. RESULTS: Among 78 Gram-positive and partially acid-fast bacilli... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase chain reaction; Rhodococcus equi; Mycolic acids. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500001 |
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| Sgarioni,Sônia Aparecida; Hirata,Rosario Dominguez Crespo; Hiroyuki Hirata,Mario; Leite,Clarice Queico Fujimura; Prince,KarinaAndrade de; Leite,Sergio Roberto de Andrade; Vedovello Filho,Dirceu; Siqueira,Vera Lucia Dias; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti. |
| Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-tuberculous mycobacteria; Milk; Mycobacterium; PCR-PRA; Mycolic acids analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046 |
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