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| Seychelles, Laurent H.; Doiron, Kim; Audet, Celine; Tremblay, Rejean; Pernet, Fabrice; Lemarchand, Karine. |
| Rotifers (Brachionus plicatilis), commonly used at first feeding in commercial fish hatcheries, carry a large bacteria load. Because they are relatively poor in essential fatty acids, it is common practice to enrich them with fatty acids, including arachidonic acid (AA). This study aims to determine whether prey enrichment with AA may act as a prebiotic and modify the microbial community composition either in AA-enriched rotifer cultures or in larval-rearing water using winter flounder (Pseudopleuronectes americanus) as a larval fish model. AA enrichment modified the bacterial community composition in both the rotifer culture tanks and the larval-rearing tanks. We observed an increase in the number of cultivable bacteria on TCBS (thiosulfate-citrate-bile... |
| Tipo: Text |
Palavras-chave: Communauté bactérienne; Rotifères; Enrichissement en acide arachidonique; Vibrio sp.; Prébiotique; Bacterial community; Rotifers; Arachidonic acid enrichment; Vibrio sp.; Prebiotic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00137/24778/25189.pdf |
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| Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean. |
| In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of... |
| Tipo: Text |
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf |
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