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| Wang,Q; Yuan,X; Li,C; Deng,H; Wang,C. |
| ABSTRACT This study was carried to express the interferon-induced transmembrane protein 3 (IFITM3) in vitro and examine its function in inhibition of avian reovirus (ARV) replication. The recombinant prokaryotic vector expressing yellow-feathered broiler IFITM3 was successfully constructed, and the recombinant protein was expressed in competent Escherichia coli BL21 cells. New Zealand white rabbits were immunized with the purified recombinant protein to prepare a polyclonal antibody, with a titer of 1:128,000. Immunohistochemistry, reverse transcription-PCR, and real-time fluorescence quantitative PCR showed that IFITM3 was distributed in the yellow-feathered broiler immune organs, and the expression of IFITM3 in bursa of Fabricius was more than in spleen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yellow-feathered broiler; Interferon; Interferon-induced transmembrane protein 3; Gene cloning; Prokaryotic expression; Avian Reovirus. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200377 |
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| Ali,S; Rajput,N; Li,C; Zhang,W; Zhou,G. |
| ABSTRACT The present study was carried out to investigate the influence of freezing-thawing cycles (0, 2, 4 and 6) on lipid oxidation and myowater contents and distribution. Nine replicates of chicken breast meat samples were used for each cycle. Lipid oxidation was determined by measuring peroxide value, and malondialdehyde (MDA) concentrations, which reflect thiobarbituric acid reactive substance (TBARS). Color was determined with a digital colorimeter. Muscle moisture contents were determined by drip loss and thawing loss, water holding capacity, and nuclear magnetic resonance (NMR). The results showed that, as the number of freeze-thaw cycles increased, meat redness decreased and MDA and peroxide values increased. Drip loss and thawing loss tended to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freeze-thaw cycles; Chicken meat; Oxidation; Drip loss; Color. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100035 |
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| Wang,Y; Yang,Q; Lin,P; Li,C; Lu,Y; Daijun,S. |
| ABSTRACT This study was conducted to evaluate the influence of supplementing tea polyphenols (TP) in diet of laying hens on yolk cholesterol content and production performance. A total of 1800 Lohmann laying hens aged 48 weeks were randomly allocated to 6 groups. Each group consisted of 6 replicates with 50 layers. The feeding experiment was 4 weeks including one-week acclimatization. Layers fed basal diet supplemented with 0, 150, 200, 250, 300 and 350 mg TP/kg diet, respectively. The results showed that average daily feed intake (ADFI), feed conversion ratio (FCR), average egg weight (AEW), laying rate and the indicators of egg quality were not significantly affected by the diet supplemented with 300 mg/kg TP (p>0.05). However, yolk cholesterol... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Laying hens; Production performance; Tea polyphenols; Yolk cholesterol content. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200302 |
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| Shan,T; Dai,P; Zhu,P; Chen,L; Wu,W; Li,Y; Li,C. |
| ABSTRACT In order to investigate the effect of organic trace minerals premix (OTM) on the reproductive performance of breeder roosters, a total of 240 San Huang roosters (23 weeks of age) were randomly divided into two treatments with six replicates of 20 roosters each. The first group (n = 120) was fed a basal diet containing an inorganic trace minerals premix (ITM) and the other group (n = 120) was fed the basal diet in which ITM was replaced by OTM. The experiment period was 22 weeks. Semen from one randomly-selected rooster per replicate was collected two weeks after the beginning of the experiment and other 10 times every two weeks. Another rooster per replicate was randomly selected at 30, 35, and 45 weeks of age, and sacrificed. Results showed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Organic trace minerals; San Huang roosters; Semen; Testes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300481 |
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| Jiang,G; Li,C; Huang,X; Zhang,X; Hu,Y; Wang,X; Wu,D; Dai,Q. |
| ABSTRACT This study was conducted to establish dietary threonine (Thr) levels for Linwu ducks, aged 4 to 8 wk. Experimental diets formulated to contain 0.56, 0.61, 0.66, 0.71, 0.76, and 0.81% Thr fed to Linwu ducks. A total of 360 healthy Linwu female ducks with similar body weight (1183.89±3.83 g) were randomly divided into six groups, with five replicates in each group, and 12 ducks in each replicate. Samples were collected at 8 wk for the determination of growth performance, carcass traits, visceral organ indices, and serum biochemical parameters. As a result of this study, Thr level had no significant influence on the final weight, the daily gain, feed/gain ratio, and average daily intake (p>0.05). Similarly, there were no significant effects of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Linwu duck; Threonine requirement; Growth performance; Carcass traits; Biochemical parameters. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200387 |
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| Abdelghani,E; Xing,W; Li,Y; Shen,D; Alsiddig,MA; Li,C. |
| ABSTRACT To investigate the effects of soy isoflavones (ISF) on the laying performance of Xueshan breeder hens at different phases of the egg production cycle, 32 weeks (wks) old local Chinese breeder hens were assigned to two groups with three replicates (750 hens/replicate) for control and treatment groups. Then every group was divided into three sub-groups following 4, 12, and 20 wks administration of control or ISF diet. General Linear Model procedure was used (2× 3), and treatments were considered of two factors (diets and time) with different levels (control and ISF diets; 4, 12, and 20 wks of diet supply time). Basal diet with 3 mg/kg ISF was used. Performance, egg quality, organs relative weight and cholesterol level were measured at three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xueshan hens; Egg quality; Hatching traits; Organs weight; Cholesterol. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400315 |
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