|
|
|
|
|
| Cao,Qinghua; Li,Tao; Shao,Huanhuan; Tan,Xuemei; Zhang,Yizheng. |
| Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first constructed with the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectors were stable in Z. mobilis ZM4 and have 3,32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene, was clonedinto the shuttle vectors, generatingthe expressionvectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori combined with the... |
| Tipo: Journal article |
Palavras-chave: Expression vector; Gene expression; Glucoamylase; Zymomonas mobilis. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100006 |
| |
|
|
| Ma,Li-Peng; Zhao,Zhi-Chao; Li,Tao; Li,Da-Quan; Wang,Xiao-Yan; Song,Cheng-Yi; Qi,Ya-Yin; Huang,Tao. |
| ABSTRACT This study aimed to investigate the roles of miRNA in the development of ovarian follicles in sows with high ovulation rate. The miRNA expression profiles in middle-size ovarian follicles between Meishan and Duroc sows were characterized by Affymetrix miRNA GeneChip. Seven differentially expressed miRNA were identified. KEGG pathways analyzed the predicted target genes of differentially expressed miRNA by using the DAVID website. The MAPK signaling pathway may affect the process of ovarian follicle development. Our study suggested that miR-21, miR-199a, miR-708, and miR-4334 might play a vital role in the development of ovarian follicles of sow. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Differentially expressed miRNA; Follicular phase; Middle-sized follicles; Sow. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100310 |
| |
|
|
|
