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| Li,X; Liu,L; Li,Q; Xu,G; Zheng,J. |
| ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in environmental samples collected in layer farms of different sizes, and to evaluate SE contamination status in four main egg-production provinces of northern China. After specificity retrieval using Primer-BLAST against the NCBI database, three SE specific oligonucleotide primers were selected as candidate primers. The primers targeting Prot6e gene were adopted and primers targeting Sdf I were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Environment; Layer farm; PCR; Salmonella Enteritidis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300377 |
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| Li,X; Su,S; Cui,N; Zhou,H; Liu,X; Cui,Z. |
| ABSTRACT Marek’s disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101∆LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens to GX0101ΔLTR remains unclear. The current study aimed at identifying the genes and pathways involved in the response to GX0101ΔLTR virus infection in specific pathogen-free chicken embryo fibroblast cells using global transcriptome analysis. A total of 1,633 genes associated with GX0101ΔLTR infection were identified. Functional analysis showed that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene profiling; Chicken; CEF cell; GX0101ΔLTR MDV.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000200179 |
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