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Li,Xinxin; Wang,Jiwen; Liu,Hehe; Wang,Haohan; Sun,Lingli; Yang,Chao; Li,Liang; Zheng,Yi. |
Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in... |
Tipo: Journal article |
Palavras-chave: Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 |
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Wang,Haohan; Li,Xinxin; Liu,Hehe; Sun,Lingli; Zhang,Rongping; Li,Liang; Wangding,Mincheng; Wang,Jiwen. |
Abstract As a critical transcription factor, Six1 plays an important role in the regulation of myogenesis and muscle development. However, little is known about its regulatory mechanism associated with muscular protein synthesis. The objective of this study was to investigate the effects of overexpression ofSix1 on the expression of key protein metabolism-related genes in duck myoblasts. Through an experimental model where duck myoblasts were transfected with a pEGFP-duSix1 construct, we found that overexpression of duckSix1 could enhance cell proliferation activity and increase mRNA expression levels of key genes involved in the PI3K/Akt/mTOR signaling pathway, while the expression of FOXO1, MuRF1and MAFbx was not significantly altered, indicating... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck; Six1; Protein metabolism signaling; MTOR; PEGFP-duSix1. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100151 |
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