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| González,Ester Teresa; Bonzo,Estela Beatriz; Echeverría,María Gabriela; Licursi,María; Etcheverrigaray,María Elisa. |
| Bovine Leukemia Virus (BLV) is the etiologic agent of Enzootic Bovine Leukosis, a retrovirus exogenous to the bovine species. Once infected, there is no detectable viraemia but instead there is a strong and persistent immunological response to BLV structural proteins, essentially the gp51 envelope glycoprotein and the mayor core protein p24. We describe the test procedure of an indirect ELISA (I-ELISA) using polyclonal reagents for the detection of antibodies to BLV. For comparison, the sera were simultaneously tested by agar gel immunodiffussion (AGID) test, which is currently used as diagnostic standard for BLV infection. The antigen applied does not require a high degree of purification and the data from the analysis of the negative sera showed that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Leukaemia; Indirect ELISA; Seroepidemiology. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100007 |
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| González,Teresa; Licursi,María; Bonzo,Estela. |
| Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine leukemia; ELISA evaluation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100001 |
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