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Differentiation of BP-type baculovirus strains using in situ hybridization ArchiMer
Durand, S; Lightner, Dv; Bonami, Jr.
BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only...
Tipo: Text Palavras-chave: Baculovirus; BP; In situ hybridization; Nonradioactive probes; Shrimp.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00447/55821/62253.pdf
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Traceability of aquatic animals ArchiMer
Hastein, T; Hill, Bj; Berthe, Franck; Lightner, Dv.
Effective methods of traceability are urgently required for use in research as weil as in different types of aquaculture operations and to control trade in aquatic animais and praducts. In regard to the marking of fish, many different tagging methods have been described and the method to be used depends on the purpose and need for tagging. In contrast, for molluscs and crustaceans, only a few methods of marking such animais have been described, due to the practical difficulties. The authors first describe the different methods for tracing fish and fishery products, by means of external tags, such as Floy tags, Carlin tags and passive integrated transponder tags; chemical marking using inorganic substances su ch as silver nitrate or potassium nitrate,...
Tipo: Text Palavras-chave: Aquatic animals; Crustacea; Fish; Genetic markers; Molluscs; Tags; Traceability.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-2701.pdf
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